p. 2
Light Path, Channels, Acquisition Mode
With one track checked, in fast settings (512x512, speed 8, no averaging) click Continuous to focus.
Optimize each channel separately by checking Range Indicator and setting pinhole (1 Airy unit) detector
gain, laser power, offset, digital gain.
Check all channels and set acquisition parameters: zoom, pixel number, scanning speed, averaging.
After setting zoom press “optimum” so that the system will calculate number of pixels according to the
optimal Nyquist number for the given objective. If scanning time is too high you can consider saving time
by:
lowering pixel dwell time.
setting scanning direction to meander instead of comb
decreasing number of averaging iterations
using frame instead of line mode
Press Snap to acquire image
For multidimensional images check the desired dimension (Z, time, tile) and after setting the relevant
parameters click Start Experiment
See instructions booklet for more details
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Saving images
Save image as CZI filetype in D:\LSM 880 USERS DATA\PI name\user name\mm-yyyy
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Data backup
Data are copied automatically every night, if computer is on and connected to the internet.
PLEASE do not use any type of USB flash drive to retrieve your data.
There are computers with active scanning anti-virus software at the Computerized Analysis Room,
connected to the servers.
Ending session
Before you finish please make sure the next user is coming.
If yes:
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Close the ZEN software
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Log off your reservation at BookItLab
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clean oil immersion objectives with lens tissue dipped in petroleum ether, twice
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clean water dipping objectives by dipping in DDW (e.g., in 35mm dish)
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Leave nosepiece at 10x objective lens
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Center the stage
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Leave surfaces clean
If no user is coming in the next two hours, also, in this order:
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Turn off the lasers in the software
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Close the ZEN software
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Log off your reservation in BookItLab
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Switch off 3
2 in the switch box
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Wait until the Lasos fan shuts off and only then switch off
1
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Cover microscope
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Turn off air condition
Do not turn off computer unless you are the last user before the weekend.
Please do not use any type of USB drive to copy your data.
