OPTO-EDU A16.2615-L Instruction Manual Download

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A16.2615-L

Inverted LED Fluorescent Microscope

Instruction Manual

To ensure the safety and obtain satisfactory performance, please study this instruction manual thoroughly be

fore start to use the instrument.

Summary of Contents for A16.2615-L

Page 1: ...6 2615 L Inverted LED Fluorescent Microscope Instruction Manual To ensure the safety and obtain satisfactory performance please study this instruction manual thoroughly be fore start to use the instrument ...

Page 2: ...must be cooled off completely before proceeding please see Fig 2 Loosening screw and replace bad bulb with required bulb The power supply must be cut off before bulb replacement 3 Microscope must be placed on stable and horizontal table 4 Only use our power line wrong power line can t guarantee instrument safety and performance 5 The microscope ground terminal must be tightly connected with plug g...

Page 3: ...d not be in direct sunlight high temperature or high humidity and dusty avoid violent shaky b Tension for coarse focusing knob can adjustable c Please leave enough space 10cm for ventilation d Please follow Fig 1 to carry microscope Firstly take away sample filters and round stage parts avoid damage If the microscope is leaning slightly not in good position rubber gasket may be fall off 2 MAINTENA...

Page 4: ...echanical Stage 10 3 5 Condenser 10 3 6 Fluorescence Attachment 11 3 6 1 Mechanism of Fluorescence Production 11 3 6 2 Parts Name 13 3 6 3 Fluorescence Observation Processes 13 3 6 4 Mercury Lamp Power House 14 3 6 5 Replacement of Mercury Bulb 15 3 6 6 Replacement of Mercury Lamp Fuse 16 4 Trouble Shooting 17 5 Installation 18 5 1 Installation Diagram 18 5 2 Installation Steps 19 5 2 1 Installati...

Page 5: ...epiece 2 Seidentopf Trinocular Head 3 Main Body 4 Adaptor for DSLR Camera 5 Coaxial Coarse and Fine Focusing Knob 6 Nosepiece 7 Attached Mechanical Stage 8 Fixed Stage 9 Turnable Phase Contrast Condenser 10 Lamphouse 1 Parts Name ...

Page 6: ...1 Adaptor for DSLR Camera 12 Coarse Fine Focusing Knobs 13 Fastening Screw for DSLR Camera Adaptor 14 Culture Dish Holder 15 Trinocular Switch Lever 16 Seidentopf Trinocular Head 17 Aperture Diaphragm Lever 18 Eyepiece 19 Fastening Screw for Lamphouse 20 Field Diaphragm Lever 21 Adjustable Knob for lamp bulb 22 Attachable Mechanical Stage 23 Handle 24 Lamphouse Plug 25 Power Socket with fuse holde...

Page 7: ...ical path Focusing sample Adjusting brightness Adjusting interpupilary distance and diopter 2 Observation Steps Adjusting lamp filament position Adjusting field diaphragm Putting objective in optical path and focusing Adjusting aperture diaphragm Turn filter into optical path Adjusting brightness Observation ...

Page 8: ...eone closing to it the bulb will turn to bright again Fig 5 Power Switch 3 1 2 Aperture Diaphragm Fig 6 Gently moving diaphragm lever to adjust aperture diaphragm for best image contrast Fig 6 Adjustment of Aperture Diaphragm and Placement Fig 7 Adjustment of Field Diaphragm and filament Of filter 3 1 3 Adjustment of Field Diaphragm and Lamp Filament a Adjust field diaphragm lever 1 to make diaphr...

Page 9: ...ion Fig 8 a Coarse focusing knob tension can be adjustable hold adjustable ring and rotate it Anti clockwise direction is decrease clockwise direction is increase b If stage is decreasing automatically the sample is deviating from the focusing point means tension too low clockwise rotating adjustable ring to increase tension Fig 8 Tension Adjustment For Coarse Focusing Knob Don t rotate coarse and...

Page 10: ...g the sample by coarse and fine focusing knob b With left eye to observation by left eyepiece focusing the sample by coarse and fine focusing knob 3 3 3 Locking Eyepiece Eyepiece can be tighten by using thumb screw Fig 11 If want to change eyepiece must unscrew it at first Fig 11 Locking Eyepiece 3 4 1 Fixed Stage a Insert standard round plate in stage b Install the clamps 2 on the stage by screw ...

Page 11: ...eded and fixed with clamps Fig 13 Mechanical Stage According to culture dish height to decide whether to disassemble the condenser a Rotate liftable knob 2 to lower phase contrast condenser b Unscrew thumb screw to disassemble phase contrast condenser 5 c Whe removing the condenser please hold the condenser with hand in case of falling and damage d Knurled screw 3 is condenser centring screw conde...

Page 12: ... the principle Now use blue ray as example B light the wavelength of 490nm when the light is emitted from the DC mercury lamp after condenser and stimulated color filter get the exciting light wavelength of 490 nm or so then through color spectroscope reflection into the objective color spectroscope has the nature of selective reflection in a certain spectrum area It will completely reflect all li...

Page 13: ...escence imaging by objective gathering and through the color spectroscope with a small amount of under 490 nm exciting light is reflected back to the light source and not through and cut off filter cutoff wavelength less than 525 nm light pure fluorescent light beam for observation and photography Fluorescent Attachment Diagram 1 Mercury Lamp 2 Condenser 3 Excitation Filter 4 Specimen 5 Objective ...

Page 14: ...mera 24 C mount 3 6 3 Fluorescence Observation Processes According to bright field observation methods to adjust microscope take following steps to make observation Put mercury lamp power supply power plug into the power socket please firstly check the power supply voltage whether conform to the requirements of the instrument and supply voltage Open the mercury lamp power switch mercury lamp power...

Page 15: ...ble time 2 minutes Fig 17 Adjustment For Mercury Lamp House 1 Condenser Adjusting Knob 2 Vertical Adjusting Knob for Mercury lamp 3 Horizontal Adjusting Knob for Mercury lamp b Use Of Power Supply Determine the input voltage is conform to the requirements of the instrument can open the main power switch open the indicator lights on the rear panel is light indicating the power switch on Gently pres...

Page 16: ...move the old mercury lamp change new mercury lamp then tighten the two fixed screw will recover lamp holder open screw tightening the mercury lamp holder Then according to the mentioned methods for mercury lamp in front of the center Fig 20 Replacement Of Mercury Lamp Don t remove mercury lamp under the condition of electricity from inside the box Danger Mercury lamp must be replaced after cooling...

Page 17: ... 6 6 Replacement of Mercury Lamp Fuse Turn off the power pull out the plug and open fuse box Mercury power supply fuse Φ5 20mm 5 0A 250V The detail steps are as follows 1 2 3 Fig 21 Replacement Of Fuse Tube ...

Page 18: ...d at right stage place Correctly placing sample and fixer it with holder f The effect of phase contrast observation image is poor Annular spot don t focus with dish holder Readjust it Culture dish bottom isn t flat Use standard culture dish 2 Mechanical Focusing Unit a Coarse focusing knob is too tight Tension too big Loosing tension adjusting ring reset again b Nosepiece glide down automatically ...

Page 19: ... g Clamps h Stage Round plate i j k Culture Dish Holder l Blood Cell Counter Holder m Attached Mechanical Stage For 96 hole Plate n Surplus Water Plate 0 LWD Infinity Plan Phase Contrast Objectives p LWD Infinity Plan Objectives q Mainbody r Adaptor for DSLR Camera s DSLR Camera 5 Installation 5 1 Installation Diagram ...

Page 20: ... of Fuse WARNING Turn off the power plug before replacement Fuse box is installed below the power socket fuse rating Φ5 20mm 5A 250V Fig 22 Replacement Of Fuse 5 2 3 Connecting Power Line a Don t curve or twine power line b Make sure the main switch at off mark O when connecting power lines c Must use three phase plug which can connect ground electrode Otherwise can t use the microscope Fig 23 Pow...

Page 21: ...ncide with dark ring phase contrast plate adjusting annular plate screw to make dark ring completely cover the bright ring d Pull the centring telescope out use eyepiece to make phase contrast observation Before Coincide After Coincide Fig 25 Phase Contrast Plate Adjustment 5 2 5 Installation of Photography Device 5 2 5 1 Video System Optional a Remove the dust cover use allen wrench to unscrew th...

Page 22: ...1 with adaptor rotating to suitable angle then tightening the locking screw 4 c Please take down the digital camera if not in use keep in dry environment Cover observation tube connector with cap for anti dust d Please read operation manual about usage of SLR digital camera and circuit connection When installing DSLR camera should remove the camera lens and change to manual position M Different ad...

Page 23: ...A16 2615 L Inverted Biological Microscope ...

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