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Quick Start Guide
Start-Up
5
Shut-Down
5
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Quick Guide to Getting an Image
6
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Place the sample on the microscope
7
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Finding the sample – transmitted light or DIC
7
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Brightfield vs. DIC
9
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Finding the sample – epi-fluorescence
9
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Finding the sample - confocal
10
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Setting Up for Confocal Microscopy
11
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Choosing the Dye
11
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Changing the Dye Selection
12
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Confocal acquisition controls.
12
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Settings for the fluorescence detectors.
13
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Additional detector controls.
13
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Laser Controls.
14
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Line Selection.
14
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Line availability, Figure 7.2.
14
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Laser Power Selector, Figure 7.3.
15
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Scroll List, Figure 7.4.
15
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Line Settings, Figure 7.5
15
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Scan Controls
16
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Scan Mode, Figure 6.1
16
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Dwell Time, Figure 6.2
16
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Scan Regions, Figure 6.3.
17
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Image Aspect Ratio, Figure 6.4.
17
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Box Size, 6.5.
17
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Box Size effect on resolution
18
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AutoHV, 6.6.
18
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Scan Area Controls
18
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Field of View, 8.1.
18
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Scan Area, 8.2.
18
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Scan Rotation, 8.3.
18
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Scan Zoom, 8.4.
19
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Reset Scan Values, 8.5.
19
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Scan Offset, 8.6.
19
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Use of Sequential, Simultaneous and Averaging.
21
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Simultaneous Capture versus Sequential Capture.
21
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Two Methods for Sequential Capture
21
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Sequential Line Capture, Figure 9.1
21
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Sequential Frame Capture, Figure 9.1
21
Olympus Fluoview-1000 User’s Guide
V.M. Bloedel Hearing Research Center, Core for Communication Research
Center on Human Development and Disability, Digital Microscopy Center
May 11, 2011
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