Zeiss LSM 880, Operating Manual

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CHAPTER 1 - SYSTEM OPERATION
ZEISS Left Tool Area and Hardware Control Tools LSM 880
242 000000-2071-464 10/2014 V_01
Single Position
Only a single well can be activated. The scanning stage will automatically move to the activated well
without the need to trigger a measurement.
To select the chamber you want to take the measurement in (only one is selectable) click on the one you
want to use. The motorized stage moves the selected chamber to the defined position under the
objective and the current well will be highlighted in blue. If no scanning stage is available, positioning has
to be done manually or by the scanners, and the
Sample carrier tab will not be available.
•
If you want to move to other positions within a well, you can do so by imaging the solution and define
positions in
LSM image .
In
Sample Carrier
only the
X,Y Stage
tab is active and the stage is used for positioning, if a
motorized stage is available. Otherwise the tab is not active.
•
Select one of the three speeds from the Speed Settings drop down box.
•
Select the step size from the Step Size (µm) scroll menu (or the input box) with the scroll arrows or
numerically.
•
The current position will be indicated in the x-Position and y-Position selection boxes.
Before clicking on a chamber you have to orient the carrier. For a non-oriented carrier, all chambers
are displayed in grey. Please orient first before you select a position. After orientation, the selected
well will appear blue.
You can also use the arrow buttons or the joystick to move the microscope stage, but in those
cases, the orientation gets lost. The selected chamber will not be highlighted any more in blue. You
can set the size of the steps to be performed via the
Step Size (µm) scroll menu (or the input box)
and the speed in the
Speed Settings
drop down menu.
Regardless which well is selected, in the result table of the FCS results window the position is
indicated as
"
1
"
.
The measurement will be triggered by pressing either the Snap or the Start Experiment action
button.