Zeiss LSM 880, Operating Manual

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CHAPTER 1 - SYSTEM OPERATION
LSM 880 Left Tool Area and Hardware Control Tools ZEISS
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5.4.19 Processing – Lightsheet Processing
Licensing Instrument
standard
 optional
LSM ELYRA
 Lightsheet Z.1
See full description of tool set for details.
The
Lightsheet Processing module allows one to process images that were acquired with the
Lightsheet Z.1 using
Dual Side
illumination and/or with the
Multiview tool
tab.
Files need to be saved prior to any Lightsheet Processing task. If this is not the case the software will ask
to save the data before proceeding.
All images and registration files resulting from Lightsheet Processing can be found in a Result
folder that is created in the folder of the original file, that was used for processing.
5.4.19.1 Dual Side Fusion
This processing task can be used for the following
datasets acquired with
Dual Side
illumination:
−
Image (Snap function)
−
Time Series
−
Z-Stacks
−
Combinations of Time Series and Z-Stacks
−
Multiview experiments as well in
combination with Time Series
When a dataset has been acquired using Dual
Side
illumination, the dataset will have the
illumination dimension saved in its metadata and
the
Dimension slider for Illumination is visible in
the Image Control area . Both illumination sides
are handled as single frames within the image.
Dual Side Fusion
provides three main options for
merging both images from the different
illumination sides into a single frame.
Choose the dataset you wish to perform the task
by mark either option (Fig. 515).
Discrete Fourier Transform Fusion :
The fusion is performed based upon the published method ("Multiple imaging axis microscopy improves
resolution for thick-sample applications", Jim Swoger, Jan Huisken, and Ernst H. K. Stelzer, OPTICS
LETTERS / Vol. 28, No. 18 / September 15, 2003).
Fine structures and details are more visible after the fusion which might be worsened when using Mean
Fusion. However, this process can create noticeable artifacts in the resulting image.
Fig. 515 Processing – Lightsheet Processing.
Dual Side Fusion