Zeiss Axio Imager User Manual Download

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Axio Imager

Upright Microscope

Operati

ng Manual

Summary of Contents for Axio Imager

Page 1: ...Axio Imager Upright Microscope Operating Manual...

Page 2: ...l as utilization and communication of its contents are not permitted Violations will entail an obligation to pay compensation All rights reserved in the event of granting of patents or registration of...

Page 3: ...ensation of ametropia when eyepiece reticles are used 35 3 4 3 Inserting the fold over eyecups 35 3 5 Setting the interpupillary distance on the binocular tube 36 3 6 Setting the viewing height 36 3 7...

Page 4: ...g the LED illuminator for transmitted light 59 3 30 Attachment lamp VIS LED for transmitted light 61 3 30 1 Installing the attachment lamp VIS LED 61 3 30 2 Adjusting the attachment lamp VIS LED 62 3...

Page 5: ...8 Operating the motorized microscope via the touchscreen of the TFT display 97 4 8 1 Screen layout 97 4 8 2 Menu structure 100 4 8 3 Home page 101 4 8 4 Favorites page 104 4 8 5 Microscope page 104 4...

Page 6: ...0 5 2 Instrument maintenance 181 5 2 1 Performing checks 181 5 2 2 Changing fuses on the manual stand 181 5 2 3 Changing fuses on the VP232 2 CAN power supply 182 5 3 Troubleshooting 183 5 4 Spares co...

Page 7: ...sed of in accordance with EU Directive 2002 96 EC WEEE This operating manual includes information and warnings that must be observed by the user The following warning and information symbols are used...

Page 8: ...lowing line voltages to be used in the ranges 100 to 127 V and 200 to 240 V AC 10 50 60 Hz without the voltage setting on the instrument having to be changed The motorized models Axio Imager M2 M2m Z2...

Page 9: ...with the dust cover when not in use Always check whether the instrument is switched off before you cover it Clogged or covered ventilation slats may lead to heat build up that will damage the instrume...

Page 10: ...contact wash the oil off with plenty of water and soap After inadvertent eye contact immediately rinse the eye with plenty of water for at least five minutes If the irritation persists consult a spec...

Page 11: ...CTION Axio Imager Warning stickers Carl Zeiss M70 2 0020 e 06 2009 430000 7344 001 11 Fig 1 3 Warning stickers on the underside of the LED illuminator Fig 1 4 Warning stickers on the attachment lamp V...

Page 12: ...arantee is provided for defects caused by natural wear wearing parts and consumables in particular and improper use The instrument manufacturer is not liable for damage caused by faulty operation negl...

Page 13: ...70 2 0020 e 06 2009 430000 7344 001 13 1 4 Overall view of Axio Imager manual 1 Eyepieces 2 Binocular tube 3 Reflected light illuminator HBO 100 4 Microscope stand manual 5 Transmitted light illuminat...

Page 14: ...4 001 M70 2 0020 e 06 2009 1 5 Overall view of Axio Imager motorized 1 Eyepieces 2 Binocular phototube 3 Reflected light illuminator HBO 100 4 Microscope stand motorized 5 Transmitted light illuminato...

Page 15: ...laboratories research clinics and medical practices Science and research colleges universities in the fields of medicine and biology Industrial applications pharmacology food technology Typical applic...

Page 16: ...is variable and can be customized to the user s requirements within the range of optionally available microscope components Coded models Axio Imager A2 A2m and A2 LED Partially motorized models Axio I...

Page 17: ...positions motorized HD DIC ACR O O O O 7 positions coded HD O O O O O O O O O Objective nosepiece 7 positions motorized HD O O O O Manual O O O O O O O O O O Modulator turret for C DIC TIC Motorized...

Page 18: ...y Internal Mechanical CAN stages Motorized O O O O O O O O O O Piezo O O O O O O O O O O Scanning stages DC stepper motors O O O O O O O O O O With manual stage O O O O O O O O O O Fast z piezo operat...

Page 19: ...INSTRUMENT DECRIPTION Axio Imager System overview of Axio Imager Carl Zeiss M70 2 0020 e 06 2009 430000 7344 001 19 2 4 System overview of Axio Imager...

Page 20: ...INSTRUMENT DECRIPTION Carl Zeiss System overview of Axio Imager Axio Imager 20 430000 7344 001 M70 2 0020 e 06 2009...

Page 21: ...INSTRUMENT DECRIPTION Axio Imager System overview of Axio Imager Carl Zeiss M70 2 0020 e 06 2009 430000 7344 001 21...

Page 22: ...INSTRUMENT DECRIPTION Carl Zeiss System overview of Axio Imager Axio Imager 22 430000 7344 001 M70 2 0020 e 06 2009...

Page 23: ...INSTRUMENT DECRIPTION Axio Imager System overview of Axio Imager Carl Zeiss M70 2 0020 e 06 2009 430000 7344 001 23...

Page 24: ...INSTRUMENT DECRIPTION Carl Zeiss System overview of Axio Imager Axio Imager 24 430000 7344 001 M70 2 0020 e 06 2009...

Page 25: ...INSTRUMENT DECRIPTION Axio Imager System overview of Axio Imager Carl Zeiss M70 2 0020 e 06 2009 430000 7344 001 25...

Page 26: ...ve color code Color ring on objective Black Brown Red Orange Yellow Green Light blue Dark blue White Magnification factor 1x 1 25x 2 5x 4x 5x 6 3x 10x 16x 20x 25x 32x 40x 50x 63x 100x 150x Objective m...

Page 27: ...0x 25 Br foc and E PL 10x 25 Br foc is 25 mm that of eyepieces W PL 10x 23 Br foc and E PL 10x 23 Br foc is 23 mm W PL and PL in the eyepiece designation refers to the excellent image flatness up to t...

Page 28: ...ans of alignment specimen 474064 0000 000 Crossline micrometer 10 100 d 26 mm Graduation length 10 mm Increments 0 1mm Graduation tolerance 0 001 mm 474066 9901 000 Net micrometer 12 5x12 5 5 10 d 26...

Page 29: ...to 1060 hPa Altitude max 2000 m Pollution degree 2 Operating data for coded Axio Imager equipped with an integrated power supply or motorized Axio Imager using the VP232 2 external power supply Operat...

Page 30: ...nt of light source continuous approx 0 7 to 12 V Mercury vapor short arc lamp HBO 103 W 2 Power consumption of HBO 103 W 2 100 W Axio Imager coded Stand with manual stage focusing Coarse drive 2 mm re...

Page 31: ...nser 0 9 H D Ph DIC mot with swivel type front lens achromatic aplanatic 0 9 DIC for objective magnifications Vobj 10x front lens 0 9 swiveled out for objective magnifications Vobj 10x front lens 0 9...

Page 32: ...tool bag with tools The following components are factory installed to the microscope stand Mechanical stage specimen holder objectives polarizer filter holder reflected light illumination and adjusti...

Page 33: ...otorized stand as described below Use an SW 3 ball headed screwdriver to undo clamping screw 3 2 4 and remove the tube 3 2 2 upwards Place dust cap 3 2 3 for tube lens protection on the dovetail of th...

Page 34: ...tube lens with its dovetail into the mount of the tube lens turret 3 3 6 and tighten clamping screw 3 3 7 3 4 Inserting eyepieces and auxiliary microscope Remove both dust caps 3 4 1 and 4 from the bi...

Page 35: ...e focusing lens of the adjustable eyepiece to focus on the line figure of the eyepiece reticle Viewing through the eyepiece use the focusing drive to focus on the microscope image of a specimen placed...

Page 36: ...e interpupillary distance is correct when you see only one round image while looking through the two eyepieces 3 6 Setting the viewing height The viewing height can be adjusted to individual requireme...

Page 37: ...with photomicrographic devices please pay attention to the corresponding manuals of the cameras as well Fix the camera adapter 60N 3 8 1 2 to the camera Remove the dust cap from the camera port Please...

Page 38: ...00 1095 168 3 9 Attaching or changing the condenser Turn coarse focusing drive to move the stage carrier to the upper stop position Turn height control 3 10 1 to move condenser carrier 3 10 2 down Sli...

Page 39: ...o prevent damage of the iris blades Use the tool to remove cover 3 12 1 or unscrew it if cover is at the bottom Turn turret disk until the DIC prism to be replaced appears in the blank aperture 3 12 4...

Page 40: ...tor modules insert them as described in Section 3 19 If the instrument is to be transported to another location the reflector turret must be replaced by the cover part provided with a grip recess 3 12...

Page 41: ...e stage carrier dovetail guide Lower stop screw 3 16 5 When employing the reflected light trans mitted light stage carrier with condenser carrier 430707 0000 000 use a slot head screwdriver to screw t...

Page 42: ...stage carrier see Section 3 13 Loosen the four locking screws 3 17 1 to 4 of the dovetail guide Move the guide plate 3 17 5 as much upward in the slots as it will go Then tighten the four screws Alway...

Page 43: ...ght and left on the stand can be provided either with a fine drive knob or a fine drive disk To change the fine drive knob or the fine drive disk proceed as follows Turn the coarse drive knob on the r...

Page 44: ...el is in the filter opening filter wheel 1 3 20 2 or filter wheel 2 3 20 4 The figure indicates the transmission in of the set filter position Set the desired position on the filter wheel Insert the c...

Page 45: ...21 5 and 6 Take care that no dirt gets onto the filter surface Screw in the retaining ring After all filters have been inserted put on the cover plate and screw it down In filter wheel 1 428301 9901...

Page 46: ...il it snaps securely into the bottom spring clips 3 22 2 of the reflector turret as well 3 19 2 Removing a module Slightly tilting the module pull it out first from the bottom spring clips and then fr...

Page 47: ...d on the barrier filter to indicate the position of the wedge angle This label should be aligned to the orientation groove 3 23 4 when you insert the barrier filter in the reflector module used This i...

Page 48: ...a screwdriver Hold both halves of the reflector module together emission half 3 25 2 and excitation half 3 25 3 turn in the position opposite to the installation position and put it down Tip up the ex...

Page 49: ...le 3 22 Activating deactivating the high performance focus When a scanning stage approx weight 4 kg is used the high performance focus must be activated to protect the focusing drive Thus specimens we...

Page 50: ...dule carrying the DOC port must be installed on the back of the stand The plug in module is to be installed by the sales technician only If the TFT display is already mounted on the motorized stand it...

Page 51: ...he stand Press the bottom part of holder Z 3 31 5 against the adjusting block so that stage carrier and bottom part of holder Z are flush Fasten bottom part of holder Z 3 31 5 with both screws 3 31 4...

Page 52: ...3 32 14 In doing so be sure to remove the protective caps from the tube sockets The pin must engage in the opening Then screw down the adapters with the sleeve nut Install and clamp two binocular tub...

Page 53: ...cussion equipment 6 Co observer tube 11 End connector R 2 Clamping screw 7 End connector L 12 Setscrew 3 Dovetail 8 Connector for power supply 13 Connecting piece R 4 Binocular tube 9 Plug 14 High lig...

Page 54: ...The power unit is set automatically to the line voltage available 3 27 2 Motorized stand Plug connecting plug 3 34 2 of power supply VP232 2 3 34 3 into the corresponding connector 3 34 1 on the rear...

Page 55: ...transmitted light socket Insert the dovetail mount of the lamp housing 3 35 9 into the corresponding socket 3 35 2 or 3 35 6 and using the SW 3 ball headed screwdriver tighten it with clamping screw 3...

Page 56: ...ed light reflected light toggle switch to the position for reflected light RL Use a power cord to connect the power connector 3 36 5 to a power outlet For reflected light use the ON OFF switch 3 36 1...

Page 57: ...eflected filament image 3 38 1 2 Fine adjustment Reattach the microscope illuminator to the microscope stand and lock it with the clamping screw Move diffusion disk for reflected or transmitted light...

Page 58: ...from connector 12 V 100 W 3 35 3 reflected light or 3 35 5 transmitted light and allow for a cool down of approximately 15 minutes Depress unlock button 3 39 3 of halogen illuminator HAL 100 3 39 1 fu...

Page 59: ...light port Installing the LED illuminator on the transmitted light port Loosen clamping screw 3 40 1 on transmitted light port 3 40 2 Remove the halogen lamp Disconnect the illuminator plug from the 1...

Page 60: ...condenser carrier 3 41 1 Move it upwards so that the bolts 3 41 5 fit into the corresponding holes on the illuminator Fasten the illuminator by means of the countersunk screws 3 41 3 located at the le...

Page 61: ...n illuminator 3 42 3 with the dovetail ring into the port 3 42 2 Use ball headed screwdriver SW 3 and the locking screw 3 42 1 to lock the illuminator firmly into position Connect the three pin illumi...

Page 62: ...tted light and remove the filter wheels for transmitted light Use 40x objective to focus on a free area of the specimen Remove the eyepiece Use the adjusting screws 3 43 1 and 2 to center the image of...

Page 63: ...3616 0000 000 or 423617 0000 000 The gray filters mounted in the 2 position filter wheels 428300 0000 000 or 428301 0000 000 are not permanently stable 3 31 2 Attaching the HBO 100 illuminator Remove...

Page 64: ...n the HBO 100 illuminator 3 47 1 via the HBO 100 transformer 4 10 2 and allow it to warm up to the operating temperature Pull out adjusting aid 3 46 1 on microscope stand The brighter arc spot of the...

Page 65: ...rol via the CAN USB converter is not possible To install the switching mirror proceed as follows If necessary remove the illuminator already mounted To do so grip the illuminator firmly and loosen the...

Page 66: ...ynchronization 6 LM set button for light manager function 7 RS 232 port 3 32 2 Motorized stand Legend to Fig 3 50 1 CAN connector 2 USB connector 3 Ethernet connector 4 RS232 connector 5 CAN connector...

Page 67: ...the threaded holes on the bottom of the stage 3 51 6 are positioned above the stage carrier openings 3 51 5 Insert four fastening screws 3 51 3 through the stage carrier from below and screw them into...

Page 68: ...ier 3 53 3 Rotate the stage to determine the maximum specimen deflection 3 53 4 tip of arrow to the eyepiece reticle Reset the two centering screws on the stage carrier 3 53 2 using one SW 1 5 Allen s...

Page 69: ...factory set to an average torque You can change this setting as follows 1 Adjusting the x direction Remove the additional sleeves 3 54 1 and 3 from the drive knobs when necessary Loosen the clamping...

Page 70: ...rotary stage Pol 3 56 5 to the front against spring loaded pin 3 56 3 lift it off the stage carrier 3 56 1 from the back and remove it upwards Retighten the screw cap 3 56 2 3 34 2 Attaching rotary s...

Page 71: ...is procedure Before centering the stage you must adjust K HLER illumination on the microscope see Section 4 9 1 For centering the stage use a contrasty specimen and an eyepiece with crossline reticle...

Page 72: ...tage use a contrasty specimen and an eyepiece with crossline reticle First turn the nosepiece to swing the non centering objective mount mount with DIC slot into the light path Center the rotary stage...

Page 73: ...1 with screws 3 59 2 to the condenser carrier making use of the provided holes 3 59 6 and 7 Hold the polarizer 3 59 4 parallel to the bottom side of the condenser carrier Using the offset adjusting le...

Page 74: ...xio Imager operation and function controls Axio Imager 74 430000 7344 001 M70 2 0020 e 06 2009 4 OPERATION 4 1 Coded Axio Imager operation and function controls Fig 4 1 Axio Imager operation and funct...

Page 75: ...sity control 15 Slot for 2 position filter wheels discrete 16 Lever for transmitted light diffusing glass 17 Control wheel for luminous field diaphragm 18 Focusing drive Fine focusing control right si...

Page 76: ...ection left with interface 60N The camera path selector may optionally be equipped with 50 beam splitter or with 100 reflecting mirror Push pull rod pushed in 100 observation through eyepieces Push pu...

Page 77: ...is needed for taking color photos on the microscope The indicator LED lights when the color temperature of 3200 K is set TL button Transmitted light shutter on off 4 1 13 Alternately opens or blocks t...

Page 78: ...2 2 To center the condenser turn the two centering screws 4 2 1 Turn the vertical control 4 2 4 to adjust the condenser condenser carrier in the Z axis The clamping screw 4 2 3 serves as stop for the...

Page 79: ...seven M27 objective mounts with slot for DIC slider or seven M27 objective mounts Objectives are quickly changed objective position by turning at the knurled ring of the objective nosepiece Reflector...

Page 80: ...itch down Transmitted light halogen illuminator ON reflected light OFF Button LM Set 4 1 37 Saves the values of the current light intensity and the optical path nosepiece position brief beep Adaptatio...

Page 81: ...OPERATION Axio Imager Coded Axio Imager operation and function controls Carl Zeiss M70 2 0020 e 06 2009 430000 7344 001 81...

Page 82: ...peration and adjustment of microscope refer to Section 4 8 7 Slot for 2 position filter wheels discrete mot 8 Motorized focusing drive coarse fine 9 Motorized objective nosepiece 10 Motorized condense...

Page 83: ...ERATION Axio Imager Axio Imager operation and function controls motorized version Carl Zeiss M70 2 0020 e 06 2009 430000 7344 001 83 Fig 4 5 Axio Imager operation and function controls motorized versi...

Page 84: ...light 4 5 4 and transmitted light 4 5 7 When using the HAL 100 halogen illuminator in the reflected light and the transmitted light path the filter wheel 2x discrete mot can be used for brightness adj...

Page 85: ...4 7 3 or 4 74 in forward or return run Motorized focusing drive coarse fine 4 5 8 on both sides of stand Operation is performed manually by turning the coarse or fine focusing controls on both sides o...

Page 86: ...st switch on the power supply VP232 2 at its on off switch 4 10 1 and then operate the on off switch on the stand Position I Adjust the desired light intensity using the light intensity control If you...

Page 87: ...2TV tube mot The 2TV tube mot 4 11 is available for all Axio Imager stands of type M2 M2m and Z2 Z2m Like all other binocular tubes this tube is to be mounted to the coupler plate and connected to th...

Page 88: ...r In addition the 2TV tube has two receptacles 4 14 1 and 2 into which P C modules 4 14 3 can be inserted One of these receptacles is factory fitted with a 100 mirror allowing 100 of the light to be d...

Page 89: ...tward and fasten coaxial drive 4 15 4 to the underside of the stage using two screws 4 15 5 Plug angled CAN bus connector 4 15 3 into one of the two sockets on the coaxial drive Place mechanical stage...

Page 90: ...he objectives are specified when you switch on the stand M2 M2m Z2 Z2m the optimum speed of stage travel is calculated directly However you may change and store this speed if necessary 4 6 3 CAN USB c...

Page 91: ...conjunction with the motorized stand If the microscope stand is inconvenient to operate from the right hand side the functions of the touch screen TFT display 4 18 3 on the docking station the pushbu...

Page 92: ...transmitted light contrast methods brightfiels phase contrast DIC darkfield polarization for the reflected light contrast methods brightfield darkfield DIC polarization and for epifluorescence When w...

Page 93: ...oltage the operator would have to use neutral density filters additionally to achieve a comparable impressionof the specimen 4 7 2 Light Manager Mode CLASSIC If the Light Manager is operated in CLASSI...

Page 94: ...be readjusted only if it is impossible to achieve the optimum illumination through the neutral density filters The computed values are automatically stored in the temporary memory of the microscope Li...

Page 95: ...swung into the light path for which the light intensity had been adjusted before already the system will automatically set the corresponding stored value The light intensity settings of halogen lamp o...

Page 96: ...ty levels the intensity of the halogen lamp is varied by changing the lamp voltage That way of course the color temperature for object observation changes as well The luminous field diaphragm is adapt...

Page 97: ...y is divided into the following main areas Fig 4 20 4 8 1 1 Navigation button bar The navigation button bar at the left edge of the screen contains buttons via which you can activate all pages The but...

Page 98: ...f the operation efficiency concept employed in all motorized Axio Imagers With its help the various contrast methods possible are set automatically and reproducibly The contrast manager can be switche...

Page 99: ...Ph DIC Reflected light Motorized reflector turret with darkfield module available C DIC TIC Circular DIC Total Interference Contrast Reflected light Motorized modulator and coded or motorized reflecto...

Page 100: ...tand type BioMed or Material does no longer have to be preselected in the user interface Fig 4 22 Menu structure On the navigation button bar the buttons of the first level appear on the very left Fig...

Page 101: ...n call all other pages via the buttons on the navigation button bar 4 23 1 The control area 4 23 2 shows the configuration identified as well as its current status All coded and motorized control elem...

Page 102: ...ile the button is depressed the stage will travel to the operating position to the upper stage stop at the most Stage moves down as long as you press this button to the lower stage stop at the most Wh...

Page 103: ...r for transmitted light TL If there is no shutter in the transmitted light path the voltage of the configured lamp will be adjusted instead Make It Visible button 4 29 3 This button serves to switch t...

Page 104: ...to the pages from where the operation of the microscope is controlled Turrets Illumination Automatic operation XYZ 4 8 5 1 Revolver page Depending on the configuration carried out under the Settings m...

Page 105: ...thod is not available for the objective concerned the system will switch to brightfield 2 Contrast tab On this page the desired contrast can be selected as a function of the reflector modules condense...

Page 106: ...lid for the contrast method determined by the reflector module have been installed the appropriate method will be set Should this be impossible the contrast manager cannot be activated at this reflect...

Page 107: ...Press the Zero button to set the mid position Turn the rear setscrew to set the shift value Each click stop position of the setscrew corresponds to a 0 05 mm change To reset the value to 0 press the Z...

Page 108: ...t though is that only the azimuth value needs to be set There is no Zero button The tab will not be available if no motorized reflector turret is installed 5 Tube lens tab Depending on the type of tub...

Page 109: ...control in transmitted light F A RL Field and aperture diaphragm control in reflected light 1 Light path tab Under the Light path tab the light path of the microscope on the imaging side is shown in a...

Page 110: ...lled independently of the remaining light path by pressing the OPEN and CLOSE buttons This tab will not be available in the absence of a light switch One of the following schematic diagrams or a simil...

Page 111: ...eyepiece Top Port and a dichroic beam splitter at the Back Port are active The active light paths are displayed in yellow inactive light paths appear in extra light gray line width 1 pixel The line w...

Page 112: ...Light path for Top Port is unknown No motorized camera path deflection at Side Port installed 2 Brightness tab This is where the lamp voltage in transmitted and reflected light and the filter wheels c...

Page 113: ...their full power completely separately or jointly The desired illuminator is selected via the On or Off buttons on the TFT the light control buttons or if there is no shutter in transmitted light even...

Page 114: ...n relation to the physical aperture of diaphragms Configuration of objectives and eyepieces available Diaphragm aperture data are presented in regard to the field of view number of the eyepieces and a...

Page 115: ...These can be simple settings or a snap with the camera or even a complex experiment The selection is made within the AxioVision software under the AxioVision Software menu item Those functions are on...

Page 116: ...controls are absent Measure tab not accessible Manual stage manual Z focus drive XYZ page is not accessible During microscope initialization the system detects whether a stage is installed Therefore t...

Page 117: ...the Z value shall be stored activate the with Z check box Press the Save Position button This will bring up the Current positions save as popup window Fig 4 52 In the window five buttons Pos 1 Pos 5...

Page 118: ...erform simple distance measurements in millimeters mm Three options are available for these measurements Distance between two manually set positions Distance between a manually set position and a defi...

Page 119: ...dere Funktionen Umschaltung DL AL die Regel bereiche der Leuchten HAL LED und VIS LED Lichtmanagerberechnung und Kontrastmanager hiervon abh ngen The Settings page provides access to the following pag...

Page 120: ...ecute the configuration procedure press the Save button To abort the procedure press the Cancel button From list button The user chooses an appropriate objective from the Preselect Magnification list...

Page 121: ...e leading six zeros or the following seven zeros after 123456 enter a hyphen or enter 1234 567 and press OK The missing zeros will be added automatically Whenever a nosepiece and objectives with autom...

Page 122: ...reflector from the list In the Resulting Configuration line you can read the current selection Press the button for RL and or TL as appropriate Press the Save button If the turret position was alread...

Page 123: ...on using Colibri as a source of illumination Once Colibri has been recognized and configured as a source of illumination an appropriate LED can be selected for each filter set or dye To do this press...

Page 124: ...tches can be set on this tab Focus Speed Up to seven buttons are displayed dependent on the actual number of positions on the nosepiece which is determined by the system during initialization and on a...

Page 125: ...ut the following steps to this end Activate the programming mode by keeping the LM Set button depressed for a while 3 s acknowledgment by a long drawn out dual beep tone While the programming mode is...

Page 126: ...generation motorized Axio Imager stands are provided with software based focus limiters which may be set by the users themselves They can be globally switched ON or OFF here Once the software limit f...

Page 127: ...operating position This has an effect on the functionality of the Load position button available on the Microscope operation page When the function is activated the focusing drive will first move int...

Page 128: ...eled with the magnification factor Proceed analogously for the other ports Splitter Up to three buttons are displayed here dependent on the equipment of the camera path deflection and the tube used Th...

Page 129: ...ected light illumination TL illumination RL illumination Motorized DIC turret Manual condenser Field of view of eyepieces Tube lens turret Reflected light configuration TL illumination The light sourc...

Page 130: ...function concerned is automatically linked with the selection of an allocated reflector module so that the user does not have to pay attention to any additional components Sources of illumination None...

Page 131: ...ings are to be made here as the positions for C DIC TIC and brightfield are always fixed Field of view of eyepieces The user chooses the eyepieces employed via the Eyepiece field of view selection pop...

Page 132: ...ully integrated in the light and contrast manager This means the following Plate When the contrast manager is used any front lens existing is swiveled out with the plate moved in front of the transmit...

Page 133: ...ected light neutral density filters as well as color and neutral density filters are offered for second generation Axio Imagers Those filters are fully integrated into the light manager functions For...

Page 134: ...lly all other options on this tab appear grayed out If a Bino Shutter is installed and dazzle protection has been activated the two other dazzle protection fields TL RL are inactive Dazzle protection...

Page 135: ...e 06 2009 430000 7344 001 135 6 Miscellaneous This tab allows you to calibrate the TFT display 4 8 6 3 Info page The Settings Info page only contains the Firmware tab This tab allows the user to ident...

Page 136: ...User selection page The User selection page contains a tab which serves to select a previously created and configured user A default user may be chosen as well This user contains the Standard settings...

Page 137: ...e knob Buttons on the right Right drive knob Docking station buttons Favorites page Selection of favorites page 1 HW Settings tab As many as ten hardware settings can be assigned to each user Each set...

Page 138: ...the OK button or discard the entry by pressing the Cancel button 2 Settings tab This tab allows the password for the user to be changed Press Change password Enter the old password Enter the new passw...

Page 139: ...n this tab the control buttons on the left hand side of the stand can be assigned Click on the Top left button pair Use the arrow buttons to select the desired assignment Acknowledge with OK or discar...

Page 140: ...se the arrow buttons to select the desired assignment Acknowledge with OK or discard with Cancel Proceeds analogously for all other button assignments 5 Docking Station tab For the assignment of the b...

Page 141: ...thout any safety query 4 8 7 3 Admin page There are two tabs on the Admin page Settings User administration 1 Settings tab The following settings can be defined on this tab Language Active illuminatio...

Page 142: ...state in which it was turned off Start page after switch on The page shown upon the start the microscope can be defined here There are two options User selection Primarily of interest for multi user...

Page 143: ...o be transferred from a user already created the Adopt user settings checkbox must be activated By pressing on factory settings you will then get to another pop up menu The user whose settings are to...

Page 144: ...ressing the Display off button turns the TFT display off Pressing that button a second time switches the TFT display on again To dim the TFT display press the Display button on the navigation bar for...

Page 145: ...transmitted light brightfield microscopy For the use of the achromatic aplanatic universal condenser 0 9 H 0 8 0 9 DF refer to Section 4 9 2 4 3 Setting transmitted light brightfield according to K HL...

Page 146: ...f view 4 114 D When changing the condenser normally the luminous field diaphragm remains centered unless the centering screws were turned To adjust the aperture diaphragm contrast remove one eyepiece...

Page 147: ...e fields the luminous field diaphragm can also be used for better contrasting by its opening being reduced by a certain range Closing it too much should be avoided in order not to worsen the evenness...

Page 148: ...darkfield attachment 1 2 1 4 Oil 424218 0000 000 3 Setting transmitted light darkfield Set K HLER illumination by analogy with transmitted light brightfield However use the objective with the highest...

Page 149: ...he luminous field diaphragm image appears almost perfectly in focus Finally match the size of the luminous field diaphragm to the size of the field of view Dark field microscopy requires specimens to...

Page 150: ...tfield front lens H into the optical path set lever 4 117 4 in position I darkfield front lens D swiveled out To swivel it out set lever in position 0 Before swiveling the darkfield front lens D in or...

Page 151: ...ding on the phase position This interference results in image contents displaying differences in intensity and color that can be perceived by the human eye 2 Instrument configuration Phase contrast ob...

Page 152: ...ince the phase stops are factory centered and centering is retained even if the universal condenser is removed from the condenser carrier and attached again To enhance the image contrast an interferen...

Page 153: ...ght Condenser with turret disk containing DIC prisms DIC I DIC II DIC III Analyzer module D DIC P C 424921 0000 000 in reflector turret Only this module may be used on the Axio Imager as it provides a...

Page 154: ...n conjunction with histological sections The same applies to acrylic glass culture chambers if the chamber bottom is made of plastic In these cases it is advisable to use chambers with bottom plates o...

Page 155: ...itted light On the modulator turret swivel in the appropriate DIC prism by pressing on knob 4 121 1 for forward rotation or on knob 4 121 2 for backward rotation of the turret Adjust the luminous fiel...

Page 156: ...they show four bright and four dark positions while being rotated through 360 between crossed polarizers Dependent on birefringence thickness as well as orientation of the specimen interference colors...

Page 157: ...sotropic direction e g of optically uniaxial or biaxial crystals is oriented parallel to the direction of observation 4 9 5 2 Determining the vibration direction n 1 Use The determination of the vibra...

Page 158: ...NE SW direction By moving compensator into the light path the specimen changes its color The kind of color change depends on the orientation of the specimen NE SW or NW SE The changes in color are bas...

Page 159: ...el to the principal vibration direction n of the compensator i e in NE SW direction the path difference of the specimen e g grayish white 150 nm and the path difference of the compen sator red 550 nm...

Page 160: ...the rotary stage Pol until the specimen is in extinction position i e it appears entirely dark In this position activate the 45 click stop Rotate the stage once by 45 so that the specimen is in diagon...

Page 161: ...thus the path difference is 5 The compensator appropriate for this application Tilting compensator K 0 30 Accessory 000000 1115 698 A dark gray as appearing interference color suggests very small path...

Page 162: ...ted light bright field according to K HLER see Section 4 9 1 3 Center rotary stage Pol and objectives if not done yet see Sections 3 34 3 3 34 4 For the further settings for the time being do not use...

Page 163: ...constantly and independently of the stage rotation in their specific interference color which depends on the material specimen thickness and orientation To obtain a contrasty image at high objective...

Page 164: ...a widely open cone In practical microscopic work this means that the condenser front lens 0 9 or 1 4 must be in the light path the aperture diaphragm fully open and the objective too should be a high...

Page 165: ...ive Check the centering of the objective by rotating the microscope stage Recenter it if necessary Turn the front push pull rod 4 126 2 to close the luminous field stop so far that only the selected s...

Page 166: ...when you rotate the stage It can be located inside or outside the imaged objective pupil depending on the position of the section With optically biaxial crystals the cross resolves into two dark hyper...

Page 167: ...trasting techniques the compensators 6x20 must be removed from the light path slot for compensators 1 Application The reflected light brightfield microscopy is the simplest and most widely used micros...

Page 168: ...nt in the exit pupil of the objective For that either pull out the adjusting aid or remove one eyepiece from the binocular tube Afterwards push the adjusting aid in again or reinsert the eyepiece Move...

Page 169: ...OPERATION Axio Imager Illumination and contrast methods Carl Zeiss M70 2 0020 e 06 2009 430000 7344 001 169 Fig 4 128 Microscope settings on Axio Imager MAT in reflected light brightfield...

Page 170: ...Epiplan Neofluar Epiplan objectives additionally labeled with HD Reflector module DF P C 6x20 compensator mount with darkfield stop for reflected light 424706 0000 000 or 4 position modulator turret...

Page 171: ...ectives Prepare the microscope as described in Section 4 9 7 for reflected light brightfield The luminous field diaphragm should just disappear from the field of view to avoid reflections Rotate the r...

Page 172: ...ne mark are facing each other 4 130 3 this corresponds approximately to the extinction position best contrast 5 Motorized four position modulator turret for circular DIC TIC Set the microscope up for...

Page 173: ...nto 6x20 compensator mount 4 129 4 or rotate turret wheel 4 130 5 of 4 position modulator turret 4 130 6 into TIC Position TIC In the field of view colored interference fringes appear Turn setscrew 4...

Page 174: ...erence is aperture dependent and decreases with increasing illumination aperture Accordingly the following correction values must be taken into account depending on the objective used Objective Correc...

Page 175: ...tire object with a homogeneous layer and then measuring the path difference If the layers or steps are transparent such as silicon dioxide on silicon the interference fringes may change their color an...

Page 176: ...anged in a reflector module FL P C together with the corresponding dichroic beam splitter 2 Instrument configuration Recommended objectives Plan Neofluar or Fluar objectives UV excitation Reflector mo...

Page 177: ...r the aperture diaphragm to the exit pupil using the two centering screws 4 134 3 and 5 if necessary Reinsert the eyepiece in the tube and close the luminous field diaphragm 4 134 7 until it is visibl...

Page 178: ...minator Epiplan Neofluar Pol EC Epiplan Neofluar Pol Epiplan Pol objectives Reflector module DIC P C or DIC Rot I P C in reflector turret or reflector module Pol P C plus analyzer slider or analyzer s...

Page 179: ...tings are required for this contrast method The Bertrand lens must be inactive rear push pull rod 4 135 6 on right side pulled out The luminous field diaphragm must be open front push pull rod 4 135 5...

Page 180: ...born oily or fatty dirt e g fingerprints and immersion oil with a cotton swab or lint free cotton cloth and the optics cleaning solution L The cleaning solution consists of 90 vol gasoline and 10 vol...

Page 181: ...2 2 Changing fuses on the manual stand Always pull the power plug before changing the fuses Should any fuse fail the cause of such failure must first be determined with any technical defect that may...

Page 182: ...d with any technical defect that may exist to be properly repaired by trained personnel The fuse compartment is located on the back of the power supply and contains two type T 4 0 A 250 V 5x20 mm fuse...

Page 183: ...inserted correctly in the filter mount Insert the filter correctly in the filter mount No DIC effect The polarizing elements are not in the light path Move the polarizing elements into the light path...

Page 184: ...r focus differences after objective change The focusing eyepieces have not been set correctly Set the focusing eyepieces to the appropriate ametropia see p 35 The objective has not been screwed in com...

Page 185: ...to the power outlet considering the power requirements of the instrument 12V 100W halogen lamp has not been installed Insert 12V 100W halogen lamp see p 58 12V 100W halogen lamp is defective Replace 1...

Page 186: ...ce Dust cover for eyepiece sockets 462981 0000 000 000000 0168 373 For closing instrument openings not in use Immersion medium Immersol 518 F Oiler 20 ml Bottle 100 ml Bottle 250 ml 444960 0000 000 44...

Page 187: ...rformed by Carl Zeiss service staff or specially authorized personnel To ensure optimum setting and trouble free function of your microscope over a longer period of time we recommend that you enter in...

Page 188: ...doc Documentation EC European Community EN European standard EMC Electromagnetic Compatibility FL Fluorescence foc focusing H Brightfield HAL Halogen lamp HBO Mercury vapor short arc lamp for fluoresc...

Page 189: ...cross flats T Slow blow fuse type TL Transmitted light TV Television UL Underwriter Laboratories UV ultraviolet VDE Verband Deutscher Elektrotechniker Association of German Electricians vis visual Vob...

Page 190: ...145 151 167 C Camera path deflection left 75 82 Camera path deflection right 76 CAN USB converter 90 Care 180 Changing fuses 182 Checks performing 181 Coarse focusing control 75 78 79 Color filter ca...

Page 191: ...76 77 82 84 Fine focusing control 75 78 79 Fine drive disk 43 Fine drive knob 43 FL attenuator discrete 75 77 82 85 Fluorescence 176 Focus linear sensor 51 Focusing drive 75 78 79 82 85 147 Front lens...

Page 192: ...ves 26 32 38 68 71 ON OFF switch 75 79 Operation 29 Operation and function controls 74 82 Orthoscopy 156 Overall view 13 14 P P C reflector module 46 Phase contrast 151 Phototube 37 Phototube 30 25 mo...

Page 193: ...tage micrometers 27 Start up 32 Stop slider 75 76 82 Switching mirror 65 Switching off 86 Switching on 86 System overview 19 T Technical data 29 TFT display 50 82 97 Toggle switch transmitted reflecte...

Page 194: ...430000 7344 001 M70 2 0020 e 06 2009 6 3 Industrial property rights Instruments instrument components or methods described in this manual are protected by patents and registered utility models US62768...

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Zeiss Axio Imager, Operating Manual

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