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Carl Zeiss
Rotary stage Pol
Axio Imager
72
430000-7344-001
M70-2-0020 e 06/2009
3.34.4
Centering objectives
The 6-position objective nosepiece Pol of the
Axio
Imager is equipped with five centering
objective mounts (without DIC slot) and one
objective mount with DIC slot (non-centering). This
mount is centered relative to the stage.
Accordingly, all objectives can be cenetered relative
to the rotary stage.
Stage centering is necessary to ensure that a
specimen feature located in the center of the field
of view does not move out while rotating the
stage. By centering all objectives, the specimen
feature remains in the center of the field of view
even after changing the objective.
•
Before centering the stage, you must adjust
KÖHLER illumination on the microscope (see
Section 4.9.1).
•
To center the stage, use a contrasty specimen
and an eyepiece with crossline reticle.
•
First turn the nosepiece to swing the non-
centering objective mount (mount with DIC slot)
into the light path. Center the rotary stage for
the non-centering objective mount as described
under 3.34.3.
•
Turn the nosepiece to move a centering objective mount into the light path.
•
Determine the position of the maximum offset of the specimen feature (3-58/
3
, origin of arrow) from
the center of the eyepiece reticle.
•
Turn the two centering screws on the objective nosepiece (3-58/
1
) with an SW 1.5 Allen key (3-58/
2
)
each to move the specimen feature by half of the arrow length towards the reticle center. Rotate the
stage again to check if the specimen feature moves off. Repeat the centering procedure, if necessary.
•
Center the other five objectives in the same manner.
To preserve this centered state it is absolutely advisable to change the objectives only by turning
the nosepiece by its knurled ring.
Fig. 3-58
Centering rotary stage and
objectives
