OPERATION
Carl Zeiss
Illumination and contrast methods
Axio Imager
146
430000-7344-001
M70-2-0020 e 06/2009
Fig. 4-114 Microscope settings on Axio Imager for transmitted-light brightfield
•
Use both centering screws (4-114/
6
1
) on the condenser carrier to center the luminous-field
diaphragm image (4-114/
C
). Then, open the diaphragm until its edge just disappears from the field of
D
).
When changing the condenser, normally the luminous-field diaphragm remains centered, unless
the centering screws were turned.
•
To adjust the aperture diaphragm (contrast), remove one eyepiece from the tube and look through the
tube with your naked eye. Adjust the aperture diaphragm (4-114/
8
) to approx. 2/3 ... 4/5 of the
diameter of the exit pupils of the objectives (4-114/
E
). In most applications, this aperture diaphragm
setting provides optimum contrast at almost ideal resolution, and is therefore the best compromise for
the human eye.
•
Insert the eyepiece back in the tube socket.
