Olympus FV1000 confocal microscope
Please
•
read and follow our
guidelines
.
•
don´t use without introduction by
ALMF
staff
•
Only switch on parts you need for your purposes
(e.g. if you don’t need the Hg lamp for epifluorescence like for overnight on confocal
microscopes)
•
report problems to
us
without delay
Laserlines:
Laser diode 405 nm (DAPI, Photoactivation, Uncaging)
Argon laser: 458, 488, 515 nm (CFP, GFP, YFP, Alexa 488, FITC)
DPSS Laser: 559 nm (RFPs, Cy3, TRITC, Alexa 555/568)
Red Diode Laser: 635 nm (Cy5, Alexa 633/647)
Switching on the system:
•
Switch on the PC (button next to floppy drive).
•
Switch on the microscope components with the power manager (Mic-
control, PSU1+2, HBO lamp if neccessary)
•
Switch on the lasers:
A) Laser combiner (FV10-MCPSU)
B) 559 nm laser if needed
1) Main switch
2) Wait until 'TEMP' is not blinking anymore
3) Turn key to 'on' position
