Nikon LABOPHOT-POL Instructions Manual Download | Manualshive

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Summary of Contents for LABOPHOT-POL

Page 1: ...Nikon Polarizing Microscope LABOPHOT POL INSTRUCTIONS NIPPON KOGAKU K K ...

Page 2: ...scope base oExchanging the lamp bulb and fuse Before replacing the lamp bulb 6V 20W or fuse turn OF F the power switch and disconnect the plug of the power source cord In such cases as of replacement do not touch the lamp bulb with bare hands im mediately after putting out the lamp it Dirt on the lens Do not leave dust dirt or finger marks on the lens surfaces They will prevent you from clear obse...

Page 3: ... eyepieces be kept in an airtight container containing desiccant oPeriodical checking To maintain the performance of the instru ment we recommend to check the instru ments periodically For details of this check contact our agency 3 CONTENTS I NOMENCLATURE 4 II ASSEMBLy 6 III PREPARATION 8 1 Interpupillary Distance Adjustment 8 2 Diopter Adjustment 8 3 Optical Path Change over in the Trinocular Eye...

Page 4: ...g Centering nosepiece CF Achromat P objective strain tree Circular graduated stage Circular graduated stage clamp screw Vernier Achromat strain free condenser Filters B GIF Dust cap 4 Eyepiece tube clamp screw Analyzer in out knob Intermediate tube clamp screw Coarse focus knob Fine focus knob Orientation plate Filter receptacle Fig 1 ...

Page 5: ...diaphragm control ring V POL stand 5 Compensator slot 1 4 A tint plate Nosepiece centering screw Specimen clip Stage rotation clamp screw Condenser centering screw Condenser aperture diaphragm control ring Dia polarizer Condenser clamp screw Field lens Brightness control dial including power switch Fig 2 ...

Page 6: ...the eyepiece CFW 10XCM into the right hand sleeve fitting the pin of the eyepiece in the right hand notch of the sleeve Into the left hand sleeve insert the CFW lOx Bottom of the Base CD 2 Achromat strain free condenser Insert the condenser into the con denser carrier fad ng the aperture number plate toward the operator Fasten the clamp screw on the left side of the carrier Aperture number plate S...

Page 7: ...n the arm of the V POL stand fitting the notch of the circular dovetai I on the end of the clamp screw Fasten the clamp screw Intermediate tube clamp screw Halogen lamp bulb 6V 20W Insert the lamp bulb with its pins into the accepting holes in the socket Note Don t touch the bulb sur face directly with the fin ger Power source cord t4 Lamp socket Insert the socket into the recep tacle on the rear ...

Page 8: ...rt the centering tools in the centering screws on the nosepiece Fig 9 For binocular observation 1 Mount the specimen on the stage Swing the objective 10 x into position and bring the specimen image into focus looking into the right hand eyepiece 2 Without manipulating the coarse and fine focus knob turn the diopter ring on the left hand eyepiece to focus on the speci men Fig 5 ____ J Fig 4 2 Diopt...

Page 9: ...ines comes to one half position of the displacement of the target Fig 10 Di OI i Fig 12 4 Remove the eyepiece from the sleeve Observing the exit pupil of objective inside rotate the dia polarizer so as to form a dark cross image on the exit pupil as shown in Fig 13 5 Centeringtbe CondenserLe I 1 Close the field diaphragm in the micro scope base to its smallest size by means of the field diaphragm ...

Page 10: ...ure for the objective Refer to P 8 7 Carry out the centering procedure for the con denser Refer to P 9 8 Bring the analyzer into the optical path 9 Swing in the objective to be usedand refocus on specimen 10 Brightness is adjusted by changing the lamp voltage Table 1 Orthoscopic Conoscopic y microscopy IN higher IN 4X or OUT wer ertrand lens OUT IN Circumscribed 70 80 of the the circumfer cal aper...

Page 11: ...opy the condenser aperture diaphragm works as a field dia phragm on the conoscopic image surface Stop down the diaphragm to such an extent that it circumscribes the circumfer ence of the field of view of the conoscopic image exit pupil of objective to shut out the stray light 5 Objectives The CF Achromat P objectives Strain free and CF eyepieces adopted in the Nikon POLARIZING MICROSCOPE LABO PHOT...

Page 12: ... cross lines direction If the protractor pin is fitted to the upper right side groove of the sleeve the cross lines will be aligned with the diagonal posi tion of the polarizaiton CF PL Projection lenses are exclusively designed for photom icrography Do not use them for observation For focusing with the observation tube of the trinocular eyepiece tube for photo 12 micrography use the eyepiece inco...

Page 13: ... polarizing microscope available in the market explain that X direction is for analyzer and V direction for polarizer Conoscopic image of this area can be observed Orthoscopic viewfield Pin hole eyepiece In the above simultaneous observation of the conoscopiC and orthoscopic images the former image may appear deviated from the orthoscopic view field center however the deviated image represents the...

Page 14: ...icroscopy light GIF For retardation measure erference ment 12 Illumination system The optical system for illumination in the LABOPHOT POL microscope is construct ed to fulfill the Koehler illumination requ irements perfectly and offers a bright un iform field without any change over manipulation Halogen lamp 6V 20W PHI LIPS 7388 is used as a light source 14 ...

Page 15: ...uire on account of their 15 insufficient brightness longer exposure times and consequently poor color reproducibility owing to the Reprocity Law Failure of film may result So when taking picture of such specimens it is recommended to use the Nikon Polarizing Microscope OPTIPHOT POL 4 Manipulation of Field and Aperture Diaphragm In photomicrography the adjustment of the field diaphragm is important...

Page 16: ...3 Table 3 Magnifications of CF PL Projection lenses Suitable for Frame Size of Photo Mask CFPLPro Large form at adapter tion lens magnification 35 mm 2 5x ro O 4 x 5 2 5x 4x 35mm 4x 6 ll 4 x 5 4x 4x E ro I 3 x 4 2 5X 4x c 6x9 2 5x 4x 5x E 4 x 5 5x 4x 3 x 4 4x 4x For photomicrography when focusing with the binocular observation tube use the CF eyepiece CF PL Projection lens and 16 CF Photo Mask eye...

Page 17: ...ng it tQ the dia gonal positio n from the extinction position and confirm that the interference color of the specimen part for measurement changes toward the lower order side by inserting the quartz wedge into the optical path If the color changes toward the higher order side rotate the stage fu rther by 90 When the fi Iter GIF is used 546nm 3 Measurement Inserting the filter GIF into the filter r...

Page 18: ... from the Y POL stand Mount the epi illuminator M on the microscope arm positioning the illumina tor nearly parallel to the arm Clamp the screw After releasing sufficiently the clamp screw on the lamp housing to which the lamp bulb 6V 20W Halogen lamp and socket is attached insert the lamp housing into the epi illuminator M and clamp the screw Connect the lamp cord to the transformer Place the epi...

Page 19: ...he analyzer graduation to 0 Slightly release the intermediate tube clamp screw on the epi illuminator M detect the orientation of the intermediate tube P to form the dark cross image on the exit pupil of objective Refer to Fig 13 and in th is position clamp the inter mediate tube P 3 Objectives Use the objectives CF M Plan Achromat P series Strain free 210 45 4 For manipulation and microscopy refe...

Page 20: ...e Refer to P 10 path Flip out Refer to P 12 18 l Swing out Refer to P 18 e AP Top lens of condenser incorrectly l Swing in to the limit 1 4 A tint plate compensator l Correct setting positioned or dust in Dirt or dust on the lens l Cleaning Condenser objective eyepiece field lens Dirt or dust on the slide l Cleaning ser Correct positioning Refer to P 9 No coverglass attached to slide l Correct use...

Page 21: ... 8 B filter not used Use B fi Iter 2 Manipulation Failures Causes Actions Upside down of slide Turn over the slide Too thick coverglass Use specified thickness O J7mm coverglass Refer to P 12 High power ob Upside down of slide Turn over the slide Too thick coverglass Use specified thickness O 17mm coverglass Refer to P 12 Eyepiece diopter not adjusted Diopter adjustment Especially when changing ov...

Page 22: ...mer for adjustment Condenser not centered I Centering Refer to P 9 Condenser aperture too much closed Open it properly Refer to P 11 mination Too low position of condenser I Correct positioning Refer to P 9 d Use 6V 20W specified Halogen bulb pHILIPS 7388 ctive l Cleaning Too low voltage Raise the voltage Not specified fuse used Use 1A 250V or O 5A 250V Lamp bulb going to be blown Replacement Lamp...

Page 23: ...ROSCOPY Eugene N Cameron John Wiley Sons Inc THE POLARIZING MICROSCOPE A F Hallimond Vickers Instruments ELECTRIC SPECIFICATIONS 100V Power source 120V 50 60Hz 220 240V Halogen lamp 6V 20W PH III PS 7388 100V 1A 250V e 120V 220 240V O 5A 250V We reserve the right to make such alterations in design as we may consider necessary in the light of experience For this reason particulars and illustrations...

Page 24: ... Nikon NIPPON KOGAKU K K Fuji Bldg 2 3 3 chome Marunouchi Chiyoda ku Tokyo 100 Japan ft03 214 5311 Telex J22601 NIKON Printed in Japan 81 12 e H E 3 ...

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