Olympus FLUOVIEW FV300 User Manual Download

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CONFOCAL LASER SCANNING

BIOLOGICAL MICROSCOPES

FV300

FLUOVIEW

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Summary of Contents for FLUOVIEW FV300

Page 1: ...CONFOCAL LASER SCANNING BIOLOGICAL MICROSCOPES FV300 FLUOVIEW...

Page 2: ...stage upright microscope platforms the FV300 offers excellent versatility as well as top class laser scanning performance Highest image quality 12 bit 2048x2048 pixel resolution with economical cost E...

Page 3: ...r introduction w Beam collimator e Polarizer r Dichromatic mirror t Excitation dichromatic mirror y XY galvanometer mirror scanners u Pupil lens i Collector Lens o Pinhole turret 0 Emission beam split...

Page 4: ...ine scanning Intensity changes may be measured over a given period of time along the length of a freely drawn line such as the trace of an axon or along a cellular junction Clip scanning By cropping t...

Page 5: ...well Once optimized steps q r can be performed easily Scanning unit set up monitor display Human colon crypt Nuclei Blue TO PRO 3 Actin Green Alexa 488 APC gene product Red Alexa 568 Christine Anderso...

Page 6: ...er glass long working distance water immersion objectives and an optional XY translation stage that moves the entire confocal microscope system while the sample and other experimental hardware remains...

Page 7: ...low chart optional time course software TIEMPO Hardware and software support to optimize the environment for FRET FRET Input output of external trigger signal The optional time course software gives c...

Page 8: ...by describing the individual steps or phases within the experiment Users can specify detailed conditions and parameters for each step This function enables users to construct complex experimental prot...

Page 9: ...h point such as XYZ coordinates the Z axis acquisition range and the detector sensitivity 2 Up to 5 X 5 adjacent fields of view registered automatically Since adjacent fields of view are registered au...

Page 10: ...on driving mechanism that enables 0 01 m step control within the BX61 BX61WI and IX81 motorized microscopes high quality continuous cross sectional images can be acquired The 3D function also provides...

Page 11: ...hysioview software Axon PC side Working conditions Clampex Ver 8 0 or later Physiolink software FV300 side Colocalization image white Colocalization By using this function to analyze multi color speci...

Page 12: ...g pseudo colors Mituyo Kinoshita Pr Kentaro Arikawa Laboratory of Neuroethology Graduate School of Integrated Science Yokohama City University Purkinje cell in the rat cerebellum FITC vesicular GABA t...

Page 13: ...ish embryo Extended focus image of 10 mx28 slice Pr Yasuhiro Kamei Pr Shunsuke Yuba Institute for Molecular and Cellular Biology Osaka University GFP labeling of Drosophila adult brain with staining o...

Page 14: ...BA625IF BA660IF BA585 640 440 LD 405 LD 458 Multi Argon 488 Multi Argon 515 Multi Argon 543 Green HeNe 568 Krypton Argon 633 Red HeNe 488 Argon SDM515 SDM505 SDM570 RDM630 SDM630 SDM560 SDM600 ECFP E...

Page 15: ...lication Real time image calculation Kalman filtering peak integration software Hardware control Laser scanning unit microscope Each image display Single channel side by side merge cropping tiling ser...

Page 16: ...Multi Ar HeNe Red Green lasers Installation stand is not included in the unit Please consult your Olympus dealer for additional laser combinations External transmitted light detector system Fluorescen...

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