Zeiss LSM 880, Operating Manual

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CHAPTER 1 - SYSTEM OPERATION
LSM 880 Center Screen Area / Image Containers - Display and Image Analysis ZEISS
10/2014 V_01 000000-2071-464 461
6.9.7 Flying Mode
The Flying mode is an interactive way to explore three-dimensional data. By pressing the mouse wheel
you can fly into and through a dataset. Moving the mouse left / right and up / down controls the flying
direction. Pressing the mouse wheel and the right mouse button at the same time allows for flying
backwards.
Once the Flying mode is activated, the rotation of the data switches modes: Whereas normally
the data turns around an imaginative point in the center of the dataset, in the fly mode the data
turns (if moved interactively with the mouse once one stops flying) around an imaginative point
" behind " the operator. Pressing the button switches back into the normal mode.
If a wheel-mouse is not available, pressing the button turns the left mouse button into the Flying
button.
The
Flying
mode is only available in graphics card accelerated render modes.
6.9.8 3D Rendering Settings in VisArt plus
Depending on the render mode selected the following adjustments can be applied to the datasets:
All settings can be saved and re-applied to other datasets by using the buttons.
−
Threshold specifies a lower threshold as a
percentage of the gray values displayed. This
decides which data enter the rendered
image. If one fluorescence channel contains
a homogeneous fluorescence signal with
high signal strength, one would set the
threshold higher in order to "protect"
signals from weaker channels from being
quenched.
−
Ambient describes a diffuse, non-directed
light source, which lets both bright and dark
structures appear darker or brighter in an
equal fashion (values on a scale between 0
and 100).
−
Specular sets a directional brightness (values on a scale between 0 and 100). Influences the
differences between dark and bright structures, which are strongest at a value of 0.
−
Shininess changes the surface gloss (on a scale between 0 and 100).
−
Ramp controls the degree of the transition from completely transparent to completely opaque.
−
Maximum influences the degree of opacity.
The settings are combined into a histogram . The x axis corresponds to the gray values and
the y axis to the degree of opacity. You can also use the mouse to interact with the histogram
curve.
Fig. 650 3D Rendering window (e.g. Surface
Advanced rendering mode)