Zeiss LSM 880, Operating Manual

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CHAPTER 1 - SYSTEM OPERATION
LSM 880 Left Tool Area and Hardware Control Tools ZEISS
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A similar procedure applies for image optimization if more than one channel is used and simultaneous
image acquisition is performed.
Make sure the suitable lasers are switched on and the channels are activated.
Pay attention to address the right channel when pinhole size, detector gain, and amplifier offset are
controlled as these are set individually for each channel.
Use the split view (see section
Split View ) to see the effect of the controls onto each channel separately.
If the acquisition is performed sequentially in with multiple tracks it is faster to activate only one track at a
time for image optimization.
5.2.23.5 How to Optimize Imaging with
Airyscan
Laser, Gain (Fig. 256)
For correct results with the Airyscan detection and
processing, overexposure needs absolutely to be
avoided.
By modifying
−
Laser power
−
and detection Gain
the detection can be set to the required level.
Fig. 256 Optimize imaging with Airyscan