ZEISS
Left Tool Area and Hardware Control Tools
LSM 880
144
000000-2071-464
10/2014 V_01
5.2.15.2
Performing a Z-Stack Using Center
Mode
Press the
Center
mode button to open the
Center
definition
panel (Fig. 194). This mode allows
setting the Range of the Z-Stack in relation to the
current
Focus Position
which is indicated as
Offset
.
In addition it is possible in the
LSM imaging
mode
to use the image of a XZ scan (line scan in
Z) for positioning the Z-Stack range. This tool sets
the first and last slice symmetrically in relation to a
defined
Center
position. One should have an idea
about the size of the stack when using this mode.
It is useful when several Z-Stacks of the same
range should be acquired at different positions
within the specimen, as the whole range of the
stack can be set to a different position at once
using the
Offset
function.
The values for number of
Slices
and
Interval
are taken over from any settings done in
First/Last
mode.
The
Offset
defines the
Center
position of the stack in relation to the
Focus Position
.
The
Slices
input box displays the number of slices of the stack. This number can be edited by typing or
using the arrow keys. Changing the value for
Slices
leaves the
Interval
untouched, but the total
Range
is adjusted.
The
Interval
input box displays the z-distance (interval) between slices in
µ
m
. This number can be edited
by typing or using the arrow keys. Changing the value for the
Interval
automatically adjusts the number
of
Slices
while adjusting the total
Range
. The
Slices
number will remain unchanged.
Pressing the
Optimal
button sets
Interval
to match the Nyquist criteria (2 fold over sampling) and
accordingly sets the number of
Slices.
The optimal interval is displayed in the button in
µ
m
.
Within the
Keep
display field (only available in
Show all
mode) you have the option to switch between
Keep
Interval
and
Keep Slice
. If
Keep Interval
is checked, the number of slices is adjusted to a new
Range
defined pressing
Set First
or
Set Last
. Likewise, when
Keep Slice
is checked, the value for the
Interval
between the slices is adjusted to a new Range defined by pressing
Set First
or
Set Last
.
The
Position (
µ
m)
input box indicates the current position of the focus, no matter if the focus drive or a
Z-Piezo
stage insert was used for positioning. The arrow keys allow to change the focus in small steps. If
the
Use Piezo
check box is ticked (only available, if a
Z-Piezo
stage is attached to the system), the
Z-
Piezo
stage insert will be used to perform all Z movements triggered from the software. If the focus drive
was used for
Set First
/
Set Last
, the system will transform this numbers for a matched
Z-Piezo
movement.
The
Fast Z Line
checkbox is only available in
LSM imaging
mode and only Line
Scan Mode
to be
selected in the
Acquisition Mode
tool. It is not possible with the
Z-Piezo
. If the check box is ticked and
Continuous
action button pressed a fast XZ scan for overviews is performed using a continuous
movement of the microscope focus. The stack size is retained and the interval is adapted depending on
the scan speed.
Fig. 194
Z-Stack tool Center mode with
Z-Stack Navigation displayed for
the LSM image mode
Summary of Contents for LSM 880
Page 1: ...LSM 880 LSM 880 NLO Operating Manual October 2014 ZEN 2 black edition...
Page 650: ......
Page 678: ......
Page 687: ......
Page 688: ......
