ZEISS
Left Tool Area and Hardware Control Tools
LSM 880
362
000000-2071-464
10/2014 V_01
Mean Fusion
:
The average intensity for each pixel is calculated using the values from both illumination sides.
Maximum Fusion
:
The intensity of each pixel of both illumination images is compared and the value with the higher
intensity is used for the resulting image.
For
Mean Fusion
and
Maximum Fusion
the checkbox
Fusion Subset in X
can be activated (Fig. 516).
With these controls it is possible to chose which area of the left and the right illumination image should
be used for fusion.
When this function is chosen, the image is displayed in the
Preview Tab
within the image container
(Fig. 517). In this preview mode the left hand image shows the original dataset and the right hand image
the preview of processing. Using the
dimension sliders
within the
image control area
the
Z-Position
,
Illumination
side and
View
can be chosen.
In
Fusion Subset in X
the currently chosen View is displayed in the
Current View
box.
With the
Left
slider or spin box the area of the left illumination side is defined. This is marked at the
slider itself with a red line, the spin box shows the pixel value of the y axis. Furthermore the cut off is
shown in the preview image with a red line for easy orientation. It is best to set the
illumination slider
of the
image control area
to 1, showing the left side illumination of the original data.
Repeat the process with the
Right
slider and input box; here the marking lines are in blue and the
illumination slider
in the
image control area
should be placed to 2, showing the right side
illumination of the original data.
The
Preview
in the image container will show the position of both sliders
Left
and
Right
as red and blue
lines respectively (Fig. 517).
To avoid a visible change in intensity along the two cut off lines, for
Mean Fusion
with
Fusion Subset
in X,
an overlap of both illumination sides should remain in the center of the image and the
Blending
slider should be used to define the number of pixels used within this overlap to crossfade both
illuminations sides.
The position of the sliders
Left
and
Right
as well as the
Blending
value must be defined for each view
individually.
Fig. 516
Dual Side Fusion – Fusion Subset in X
Summary of Contents for LSM 880
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