Zeiss LSM 880, Operating Manual

Page: 392 / 690

CHAPTER 1 - SYSTEM OPERATION
ZEISS Left Tool Area and Hardware Control Tools LSM 880
386 000000-2071-464 10/2014 V_01
5.4.21.3 ICS Correlation
•
Activate ICS correlation in the ICS tool. The
ICS correlation
menu will appear (Fig. 542).
•
Highlight the image in the image container and
press the
Select button. This will be your Input
image.
•
This function is only available in the LSM
imaging mode
A correlation image will be computed based on the
settings and displayed in the
Preview
window. As
an output image an image with the selected
average subtracted is computed and the result
presented in the
Preview
window.
•
The correlation to be computed can be selected
from the
Correlation drop down menu
(Fig. 543). For one channel recordings, only
Spatial auto-correlation (S+A)
is available, for
a two channel recording in addition Spatial
cross-correlation (S+C)
.
The way of average subtraction can be selected
from the
Remove structures drop down menu
(Fig. 544).
•
You can select between None (no average
subtraction),
Slowly moving structures
(subtraction of moving average, the frame size
of which can be selected in the
Average
frames input box), Immobile structures
(+Stack) and Immobile structures (+Plane)
(subtraction of total average and adding
average scalar of all pixels from one frame to
each corresponding frame).
Fig. 542 Processing – ICS correlation
Fig. 543 Correlation drop down menu
Fig. 544 Remove structures drop down
menu