Zeiss LSM 880, Operating Manual

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CHAPTER 1 - SYSTEM OPERATION
LSM 880 Left Tool Area and Hardware Control Tools ZEISS
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5.3.2.2 NDD Light Path (BiG.2 as NDD)
BiG.2 can be attached to the transmitted (NDD-T)
and/or reflected (NDD-R) position (not more than
two BiG.2 modules in can be used on one system
in total) up to four GaAsP detectors can be used
simultaneously to acquire up to four
autocorrelation functions (AC), one for each
channel. Each of these channels can be cross-
correlated to itself or to any of the other activated
channels (CC), but a total number of ten cross-
correlation channels is the upper limit.
Selection of dichroics and filters
From top to bottom you have the following
dichroics and filters available.
Main dichroic beam splitter (MBS):
The MBS reflects the specified laser line and allows
the resulting fluorescence spectrum to pass
through.
•
Click on the MBS icon to open a list of dichroics
(Fig. 262).
•
Select the appropriate one by a mouse click.
•
Close the list by clicking the icon again.
•
You have two major beam splitter filter wheels,
one for the visible lasers (458 to 633 nm) and
one of the invisible light (for UV, 405, 445 and
NLO laser).
Secondary dichroic beam splitter (SBS):
The SBS splits the fluorescence spectrum onto the various detection channels. The long pass (LP) filter lets
wavelengths pass above the specified border wavelength and reflects wavelengths below. The short pass
(SP) filter lets wavelengths pass below the specified border wavelength and reflects wavelengths above.
The SBS is positioned in a 45° angle to the incident beam.
The icon is not displayed, as the SBS fitler wheel is set automatically for directing the light either to the
internal detectors (3 or 32 Channel Module) or to the external detectrs BiG.2 (2 Channel Module. This is
dependent on which detectors are activated. For BiG.2 (also as NDD) the filter cube holds both, a beam
splitter and two emission filters. The filter cube has to be replaced manually.
Emission filters:
The EF is used to narrow the fluorescence spectrum. The band pass (BP) filter allows the range within the
specified border wavelengths to pass through. The long pass (LP) filter allows the range above the
specified border wavelength to pass through. The short pass (KP) filter allows the range below the
specified border wavelength to pass through. The EFs are positioned in a 90° angle to the incident beam.
Fig. 262 Light Path tool for the BiG.2 on
NDD port