Zeiss LSM 880, Operating Manual

Page: 176 / 690

CHAPTER 1 - SYSTEM OPERATION
ZEISS Left Tool Area and Hardware Control Tools LSM 880
170 000000-2071-464 10/2014 V_01
•
Activate channel 1 via the check box. You may
want to assign a different color to the channel
which will be taken over for the initial display of
the image. Click the color triangle and select
the desired color from the drop down menu
(Fig. 251).
•
Use the dye triangle to display the emission
spectrum of your dye.
•
Use the slider for the respective channel
(Fig. 249) to set the required detection band
(e.g. 566-644nm).
•
If required, deactivate all the other channels
(ChS, Ch2, transmission, R1-2) via check box.
•
Click on the icon of the main dichroic beam
splitter and select
MBS 488/561
or any other
MBS indicating the 561 line.
The beam path in the
Imaging Setup tool now displays the way the potential emission signal takes.
Channel 1 receives the red part of the spectrum. The settings are suitable for imaging dyes like
Rhodamin, TRITC or Cy3.
For overlaying fluorescence and transmitted-light images, click on the
T-PMT (Transmission) button in the
Beam Path and Channel Assignment panel.
All transmitted light applications like
−
phase contrast
−
differential interference contrast (DIC)
−
polarization contrast (Pol)
−
darkfield
can be performed if the hardware of the microscope is equipped with the necessary optics. We
recommend DIC as standard contrast because the suitable objectives have a higher transmission than the
ones for phase contrast. This enhances detection sensitivity.
In case intensity fluctuations occur e.g. with 405 nm lasers, a different attenuation level should be
chosen. In case this is not possible, a different laser line needs to be selected for the T-PMT acquisition or
track.
Fig. 251 Channel color selection