Zeiss ELYRA User Manual Download

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Wellcome Trust Centre for Human Genetics

Cellular-Imaging Microscopy Core

The Zeiss Elyra TIRF
Confocal

Abridged INSTRUCTIONS

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Summary of Contents for ELYRA

Page 1: ...Page 1 Wellcome Trust Centre for Human Genetics Cellular Imaging Microscopy Core The Zeiss Elyra TIRF Confocal Abridged INSTRUCTIONS...

Page 2: ...super resolution with temperature control Plan Apochromat 10x NA 0 3 air objective Plan Apochromat 20x objective NA 0 8 air objective Alpha Plan Apochromat 100x NA 1 46 oil objective Andor iXon DU 89...

Page 3: ...ain with the camera it s locked in software The EM Gain going from 1 to 200 goes from the equivalent of second to a 20ms to frame rate Avoid putting too much light onto the cameras EMCCD sensor The EM...

Page 4: ...r use If you are using the heated incubator at 37oC you should switch on the incubator at least 2 hours before imaging For the most stable time lapse imaging at 37oC leave the incubator on overnight T...

Page 5: ...yra microscope The tube lens can be 1x or 1 6x always use the 1 6x with super resolution If the 1x tube lens is selected you can see the shadow of the 1 6x down the microscope vanishes when the 1 6x i...

Page 6: ...n OOO appears on the display this is the lamp intensity Press Up Down to select 0 12 25 50 and 100 lamp intensity Warning things can very bright down the eyepieces even with the standard FITC TRITC Fa...

Page 7: ...ails on the operation of the Elrya TIRF super resolution microscope see the Ziess Elyra manual and in particular the Elyra Quick Guide after chapter 7 of the manual a pdf download of the Quick Guide i...

Page 8: ...ith excitation light in combination with open close filter options in Zen Black 8 Ensure that the doors and viewing panels are closed on the black stage incubator or the lasers will switch off Wait 5...

Page 9: ...y possibility of damage during start up when the XY motorised stage initialises Note The top of the 100x objective rotates so avoid accidently turning it 4 Drop the 10x objective nearer to the Load lo...

Page 10: ...ave any microscope imaging problems or queries just ask a member of the Microscopy Core staff for help There is additional help and advice on our Core web pages http www well ox ac uk microscopy WTCHG...

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