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The X-cite Series 120PC Q 

The X-Cite takes a minute or so warm up 

Use the Mode button to select three modes: 

1.

 

Press mode and when OOOO. appears on the display this is the number 
of hours the arc lamp has been on 

2.

 

Press mode and when OOO.O appears on the display this is the timer 
mode. The display is in seconds and 1/10 seconds. It times how long the 
lamp will be on in seconds. Set the time and press Start (Stop) and lamp 
will come on and the timer will count down to switch-off. 

3.

 

Press mode and when OOO appears on the display this is the lamp 
intensity. Press Up/Down to select 0, 12, 25, 50 and 100% lamp 
intensity. Warning things can very bright down the eyepieces even with 
the standard FITC/TRITC/Far red triple filter selected. 

Press Start/Stop to switch open/close the X-Cite shutter and illuminate the 
specimen. The footswitch under the microscope also activates the Start/Stop 
sequence. 

Note that the Excite 120PC Q only works with the triple filter block FSet77HE 
(for GFP/DsRed/Cy5 only and so no DAPI) when viewing down the eyepieces. 
The triple filter FSet77HE excites at 483 + 564 + 642 nm (HE) and shows all 
three fluorescence colours at the same time, although our eyes can’t see the 
far red Cy5 emission. 

 

  

 

 

Summary of Contents for ELYRA

Page 1: ...Page 1 Wellcome Trust Centre for Human Genetics Cellular Imaging Microscopy Core The Zeiss Elyra TIRF Confocal Abridged INSTRUCTIONS...

Page 2: ...super resolution with temperature control Plan Apochromat 10x NA 0 3 air objective Plan Apochromat 20x objective NA 0 8 air objective Alpha Plan Apochromat 100x NA 1 46 oil objective Andor iXon DU 89...

Page 3: ...ain with the camera it s locked in software The EM Gain going from 1 to 200 goes from the equivalent of second to a 20ms to frame rate Avoid putting too much light onto the cameras EMCCD sensor The EM...

Page 4: ...r use If you are using the heated incubator at 37oC you should switch on the incubator at least 2 hours before imaging For the most stable time lapse imaging at 37oC leave the incubator on overnight T...

Page 5: ...yra microscope The tube lens can be 1x or 1 6x always use the 1 6x with super resolution If the 1x tube lens is selected you can see the shadow of the 1 6x down the microscope vanishes when the 1 6x i...

Page 6: ...n OOO appears on the display this is the lamp intensity Press Up Down to select 0 12 25 50 and 100 lamp intensity Warning things can very bright down the eyepieces even with the standard FITC TRITC Fa...

Page 7: ...ails on the operation of the Elrya TIRF super resolution microscope see the Ziess Elyra manual and in particular the Elyra Quick Guide after chapter 7 of the manual a pdf download of the Quick Guide i...

Page 8: ...ith excitation light in combination with open close filter options in Zen Black 8 Ensure that the doors and viewing panels are closed on the black stage incubator or the lasers will switch off Wait 5...

Page 9: ...y possibility of damage during start up when the XY motorised stage initialises Note The top of the 100x objective rotates so avoid accidently turning it 4 Drop the 10x objective nearer to the Load lo...

Page 10: ...ave any microscope imaging problems or queries just ask a member of the Microscopy Core staff for help There is additional help and advice on our Core web pages http www well ox ac uk microscopy WTCHG...

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