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Introduction 

The Zeiss Elyra TIRF PALM/STORM super-resolution microscope is located in     
room 00/071, Lab 1 in the WTCHG building. 

The new Zeiss Elyra TIRF microscope has: 

 

Zeiss Observer wide-field inverted TIRF flourescence microscope 

 

X-Cite 120Q fluorescence excitation illuminator 

 

Triple dichroic filter for FITC, TRITC and Cy5 far red 

 

NewPort Vision IsoStation anti-vibration isolation table for Elyra system 

 

PALM super-resolution illumination module 

 

Piezzo fast Z stage with 100μm range 

 

Motorised XY stage 

 

Black box XL stage incubator for super-resolution with temperature 
control 

 

Plan Apochromat 10x NA 0.3 air objective 

 

Plan Apochromat 20x objective NA 0.8 air objective 

 

Alpha Plan Apochromat 100x NA 1.46 oil objective 

 

Andor iXon DU 897 EM-CCD (512x512 pixel) camera 

 

DAPI 405nm line 50mW diode laser 

 

GFP 488nm line 100mW laser 

 

DsRed 561nm line 100mW laser 

 

Imaging PC workstation for Elyra systems S.1, P.1 and PS.1 

 

30" LCD TFT PC monitor 

 

Zeiss ZEN Black image acquisition software with super-resolution 
PALM/STORM module 

 

Two hardware USB2 licence keys for Zen Black imaging software, one 
on the microscope PC and the other on the Core’s Imaging Workstation 

 

To view the Ziess image files on your office PC you will need to download and 
install Zeiss's Zen Lite program (Windows PCs only). 

 
 
 

 

Summary of Contents for ELYRA

Page 1: ...Page 1 Wellcome Trust Centre for Human Genetics Cellular Imaging Microscopy Core The Zeiss Elyra TIRF Confocal Abridged INSTRUCTIONS...

Page 2: ...super resolution with temperature control Plan Apochromat 10x NA 0 3 air objective Plan Apochromat 20x objective NA 0 8 air objective Alpha Plan Apochromat 100x NA 1 46 oil objective Andor iXon DU 89...

Page 3: ...ain with the camera it s locked in software The EM Gain going from 1 to 200 goes from the equivalent of second to a 20ms to frame rate Avoid putting too much light onto the cameras EMCCD sensor The EM...

Page 4: ...r use If you are using the heated incubator at 37oC you should switch on the incubator at least 2 hours before imaging For the most stable time lapse imaging at 37oC leave the incubator on overnight T...

Page 5: ...yra microscope The tube lens can be 1x or 1 6x always use the 1 6x with super resolution If the 1x tube lens is selected you can see the shadow of the 1 6x down the microscope vanishes when the 1 6x i...

Page 6: ...n OOO appears on the display this is the lamp intensity Press Up Down to select 0 12 25 50 and 100 lamp intensity Warning things can very bright down the eyepieces even with the standard FITC TRITC Fa...

Page 7: ...ails on the operation of the Elrya TIRF super resolution microscope see the Ziess Elyra manual and in particular the Elyra Quick Guide after chapter 7 of the manual a pdf download of the Quick Guide i...

Page 8: ...ith excitation light in combination with open close filter options in Zen Black 8 Ensure that the doors and viewing panels are closed on the black stage incubator or the lasers will switch off Wait 5...

Page 9: ...y possibility of damage during start up when the XY motorised stage initialises Note The top of the 100x objective rotates so avoid accidently turning it 4 Drop the 10x objective nearer to the Load lo...

Page 10: ...ave any microscope imaging problems or queries just ask a member of the Microscopy Core staff for help There is additional help and advice on our Core web pages http www well ox ac uk microscopy WTCHG...

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