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The two buttons on the left side by the focus knob control the reflector turret
and light path (this is far easier selected by Zen Black software on the PC).
You can switch microscope objectives via the Zeiss microscope remote LCD
station or within Zen Black software.
There is no ‘definite focus’ autofocus hardware on the Elyra microscope.
The tube lens can be 1x or 1.6x – always use the 1.6x with super-resolution. If
the 1x tube lens is selected you can see the shadow of the 1.6x down the
microscope (vanishes when the 1.6x is selected).
You can do a multi-location time lapse with an identical z stack, and say a 3x3
raster scan at each location in time-lapse.
The Elyra isn’t a confocal and the Core’s Zeiss Spinning Disk confocal system
should be more ‘gentle’ with live cells. Start with lower laser power as the
Elyra has high powered lasers (around 100mW).
