Siemens ADVIA 2120, Operator'S Manual, Page: 86 / 477

Troubleshooting the Analyzer 5-19
Cleaning the Flowcells off the Analyzer: Step 4
Flushing the Flowcell and the CFM
1. Reattach the CFM to the flowcell
assembly. Do not overtighten the
locking nut.
2. To flush each nipple separately, the
other two nipples have to be blocked
off. To do this, knot a piece of
0.020(0.5-mm) ID tubing in the
middle of its length. Attach the two
ends of the tubing to the nipples that
are not being flushed.
3. Draw 10 mL of 25%
solution of household
bleach and water into the
syringe. Connect the
syringe and adapter to the
flowcell-tube-assembly
fitting.
4. Working over a sink, carefully press the plunger to push the contents of the
syringe into the flowcell and out the open nipple.
5. Switch one end of the knotted tubing to the cleaned nipple.
6. Disconnect the syringe and the adapter, and draw 10 mL of the appropriate
fluid into the syringe. Flush the flowcell with the fluid now exiting through
the second nipple.
7. Repeat the flushing for the third nipple
8. Repeat steps 2 through 7 using water to rinse out the flowcell and the CFM.
9. Disconnect the syringe and adapter from flowcell tube assembly fitting.
Cleaning the Flowcells off the Analyzer: Step 5
Cleaning the Glass Windows of the Flowcell
1. Fold a piece of lens tissue so that one edge is slightly narrower than the
optical glass window.
CAUTION
To avoid getting material from your hands onto the optical surfaces (glass
windows), do not touch the area of lens paper that will come in contact with
the glass windows.
2. Soak this edge of the tissue paper with methanol.