Siemens ADVIA 2120, Operator'S Manual, Page: 298 / 477

Methods 8-29
The following PEROX cytograms were obtained from a representative patient
specimen.
1 Noise
2 Nucleated Red Blood Cells
3 Platelet Clumps
4 Lymphocytes and Basophils
5 Large Unstained Cells
6 Monocytes
7 Neutrophils
8 Eosinophils
Noise
This area contains Platelets, RBC stroma, and debris that are excluded from the
Total White Count (WBCP) and WBC differential.
Nucleated Red Blood Cells
If present, nucleated red blood cells appear in this area because of their small size
and lack of peroxidase staining. The system uses data from the perox channel and
the baso channel to derive the Nucleated Red Blood Cell (NRBC) count
(%NRBC and #NRBC). If NRBCs are present, the system corrects the WBC
results to account for them.
Platelets Clumps
If present, platelet clumps appear in this area. The platelet clump count is not
included in the WBCP and differential, but it is monitored for flagging purposes.
Lymphocyte and Basophil Cluster
Lymphocytes and Basophils appear in the same cluster because they have similar
size and no peroxidase activity.
LUC Cluster
The LUC cluster contains Large Unstained Cells (atypical lymphocytes, blasts,
and other abnormal cells).
Monocyte Cluster
Monocytes are medium-sized cells with some peroxidase activity.
Neutrophil Cluster
Neutrophils are large cells with strong peroxidase activity.
Eosinophil Cluster
Eosinophils have the most peroxidase activity of all the white blood cell types
and are large cells, approximately the same size as neutrophils.
Eosinophils stain so intensely that they absorb light that would otherwise be
scattered. Consequently, eosinophils appear somewhat smaller than neutrophils
and can be separated from them.