Siemens ADVIA 2120, Operator'S Manual, Page: 297 / 477

8-28 Methods
Perox Y Histogram
1 Noise
2 Lymphocytes
3 Large Unstained Cells (LUC)
PEROX Y histogram
displays the light scattering
values for all events,
including those from the
noise area.
Increasing channel numbers
on the PEROX Y histogram
correspond to greater cell
size (volume).
Noise–Lymph Histogram
The Noise-Lymph histogram provides the light scattering values for the noise,
lymphocyte, and large unstained cells (LUC) areas.
The Noise-Lymph histogram is used to derive the following parameters:
•
The mode channel of the noise population on the y axis (1)
•
The peak channel (Lymph Mode) of the lymphocyte population on the y axis
(2)
•
The valley channel (Noise-Lymph Valley) that separates the noise and
lymphocyte populations (3)
•
The relative depth of the valley as expressed by d/D (PEROX d/D).
A Noise
B Lymphocytes
PEROX Cytogram
The PEROX cytogram is divided into 100 counting channels on each axis. The
cells absorb light proportional to the amount of peroxidase stain present, and this
is represented on the x axis. Cells scatter light proportional to their size, and this
is represented on the y axis.
When the light scatter and absorption data are plotted, distinct populations or
clusters are formed.
Cluster analysis identifies each population based on its position, area, and
density, and then the number of cells in each population is processed. The lines
that separate the different cell populations are calculated by the software on a
sample-by-sample basis.