Siemens ADVIA 2120, Operator'S Manual, Page: 295 / 477

8-26 Methods
ADVIA 2120/2120i PEROX 2
NOTE:
For more detailed information on the contents of ADVIA 2120/2120i
PEROX 2 reagent, please see Chapter 8.
ADVIA 2120/2120i PEROX 2 and ADVIA 2120/2120i PEROX 3 are added to
the peroxidase reaction chamber in the second reagent addition.
The 4-Chloro-1-naphthol in ADVIA 2120/2120i PEROX 2 serves as a substrate
that enables the hydrogen peroxide in ADVIA 2120/2120i PEROX 3 to form a
dark precipitate at endogenous sites of peroxidase activity in the granules of
white blood cells, as described by the following equation:
ADVIA 2120/2120i PEROX 3
NOTE:
For more detailed information on the contents of ADVIA 2120/2120i
PEROX 3 reagent, please see Chapter 8.
ADVIA 2120/2120i PEROX 2 and ADVIA 2120/2120i PEROX 3 are added to
the peroxidase reaction chamber in the second reagent addition.
The 4-Chloro-1-naphthol in ADVIA 2120/2120i PEROX 2 serves as a substrate
that enables the hydrogen peroxide in ADVIA 2120/2120i PEROX 3 to form a
dark precipitate at endogenous sites of peroxidase activity in the granules of
white blood cells, as described by the following equation:
ADVIA 2120/2120i PEROX SHEATH
NOTE:
For more detailed information on the contents of ADVIA 2120/2120i
PEROX SHEATH reagent, please see Chapter 8.
ADVIA 2120/2120i PEROX SHEATH has three important functions:
•
To constrict the sample stream so that only one cell at a time can pass
through the viewing area.
•
To prevent contact between the sample stream and the flowcell walls. This
prevents clogs and staining of the flowcell.
•
To provide an optically transparent medium through which the sample
stream can be clearly focused.
Measurement
A constant volume of the cell suspension from the Perox reaction chamber passes
through the flowcell where the absorption (peroxidase staining) and the forward
light-scattering (size) signatures of all white blood cells are measured.