Siemens ADVIA 2120, Operator'S Manual, Page: 325 / 477

8-56 Methods
NRBC Analysis
NRBC Method Description
The ADVIA 22120/2120i/22120/2120ii NRBC analysis method reports NRBC
counts for whole blood samples with either 200 or more nRBC/
μ
L or with at
least 2% nRBCs with a WBC count of at least 3000/
μ
L.
The method reports both an absolute nRBC count (10 9 /L) and a percentage
count (#NRBC/100 WBC). It also corrects the WBC count for nRBC,
recalculates the WBC differential and %MN and %PMN. The uncorrected counts
are also available and are designated by a lower case “u”.
NOTE
:
NRBC results are available only for samples processed in CBC/Diff or
CBC/Diff / Retic mode.
PEROX Cytogram
with location of nRBC and Unstained
line
BASO Cytogram
with location of nRBC
The method identifies nRBCs by nuclear size in the Peroxidase channel, and by
nuclear density in the Basophil/Lobularity channel.
In the unstained region of the Peroxidase Channel cytogram, nRBC nuclei are
located between the noise and lymphocytes. They often form distinct
populations, which are analyzed to produce counts.
In the Basophil/Lobularity channel cytogram, nRBC nuclei are located in the
polymorphonuclear region, rather than in the mononuclear region, because they
are denser than lymphocyte or monocyte nuclei. Since they are not the nuclei of
polymorphonuclear cells, the difference between the number of nuclei in this
region and the sum of neutrophils and eosinophils in the Peroxidase Channel may
equal the NRBC count. This difference is called the "Barox" (
Ba
so / Pe
rox
)
count.