Siemens ADVIA 2120, Operator'S Manual, Page: 335 / 477

8-66 Methods
Using the Mie theory of scattering for homogeneous spheres , the low-angle and
high-angle light scatter signals for each cell are transformed into volume and
hemoglobin concentration values. RBCs have cell volumes between 30 fL and
180 fL, and hemoglobin concentrations between 19 g/dL and 49 g/dL.
Bibliography
Tycko DH, Metz MH, Epstein EA, Grinbaum: Flow-cytometric light scattering
measurement of red blood cell volume and hemoglobin concentration.
Applied
Optics 24(9)
:1355-1365 (1985)
Calculating Retic Parameters
Reported Parameters
Parameter Explanation
#Retic RBC x (%Retic
÷
100) x 1000
%Retic 100 x (RETIC Count
÷
#RTC Gated Cells) x %
RETIC Cal Factor
CHr Mean of the RETIC CH histogram for the
reticulocyte population
System Parameters
The following parameters are for research or laboratory use only and are not for
patient reporting:
Parameter Explanation
RTC RBC RBC Count
RTC Flatness
Sum of theSquared Differences
Mean Cell Counting Rate 9
×
RTC % Dead Time 100 x (Measured Dead Time
÷
Measured Sample Time)