Siemens ADVIA 2120, Operator'S Manual, Page: 399 / 477

9-58 Regulatory Information
Sample Handling
Sample Collection
Collect whole blood in an evacuated blood collection tube containing EDTA as
an anticoagulant. Blood samples should be refrigerated at a temperature from 2°C
to 8°C, if they will not be analyzed within eight (8) hours of phlebotomy. If a
specimen has been refrigerated, allow it to equilibrate slowly to room
temperature (15°C to 30°C) before mixing.
Sample Dilution
Dilution of whole-blood specimens for RETIC analysis is not recommended.
Sample Stability
The effect of the aging of blood was studied over a 72-hour period on the
ADVIA 120 Hematology System.
Two whole-blood specimens drawn from 15 normal, apparently healthy donors
were assayed shortly after phlebotomy and then again at intervals of 8, 24, 36,
48, 56, and 72 hours.
One of the whole blood specimens from each pair was stored at room
temperature while the corresponding specimen was stored at 2°C to 8°C in
capped blood collection tubes that contained EDTA as the anticoagulant.
The results indicate that RETIC parameters are stable within 2 standard
deviations (within run precision) of the initial recovery for the specified time
interval. The stability of calculated parameters is limited to the stability of the
least stable primary parameter.
Parameter Room Temperature
Stability (hours)
Refrigerated Temperature
Stability (hours)
%RETIC 24 72
CHr 24 72
Materials Required but not Provided
For the ADVIA 2120/2120i Hematology System, the other materials required to
perform the ADVIA 120 reticulocyte method are the various control materials,
calibrators, ADVIA OPTIpoint, and other reagents or equipment specified in the
"Methods Introduction" section.
Procedure
To run samples on your ADVIA 2120/2120i Hematology System, refer to the
Daily Routine.