Siemens ADVIA 2120, Operator'S Manual, Page: 311 / 477

8-42 Methods
RBC HC Histogram
The RBC hemoglobin concentration (RBC HC) histogram represents the
distribution of red blood cells by cellular hemoglobin concentration. The
histogram has a range of 0 g/dL to 50 g/dL.
RBC HC histogram (normal sample)
1 Hypochromic region
2 Normochromic region
3 Hyperchromic region
4 28 g/dL marker
5 41 g/dL marker
Normal samples have a bell-curve shaped Hgb concentration distribution with a
mean channel between 28 g/dL and 41 g/dL. The cell hemoglobin concentration
mean (CHCM) and the hemoglobin distribution width (HDW) are obtained from
this histogram. The CHCM is the mean of the RBC HC histogram. The HDW is
the standard deviation of the RBC HC histogram. The CHCM calibration factor
has been applied to the displayed data.
RBC HC histogram (hypochromic sample)
1 Hypochromic region
2 Normochromic region
3 Hyperchromic region
4 28 g/dL marker
5 41 g/dL marker
In samples with increased numbers of hypochromic RBCs, the histogram curve
shifts to the left, indicating an increase in the percentage of the cells with
hemoglobin concentrations less than 28 g/dL.
RBC HC histogram (hyperchromic sample)
1 Hypochromic region
2 Normochromic region
3 Hyperchromic region
4 28 g/dL marker
5 41 g/dL marker