Siemens ADVIA 2120, Operator'S Manual, Page: 11 / 477

Welcome to the ADVIA 2120/2120i Hematology System 1-7
The baso/lobularity reaction chamber ( 2 ) is temperature controlled. The mixture
of sample and reagent is heated to achieve the desired cytochemical reaction.
The Hgb reaction chamber ( 1 ) serves as an optical cuvette in which the
hemoglobin measurement is read.
The RBC/Plt and retic reaction chambers ( 3 ) and ( 4 ) serve as containers in which
the reagents and samples are mixed for the desired cytochemical reaction.
Optical Assemblies
1 2 3
The perox optical assembly ( 1 ) directs light from a tungsten-halogen lamp to the
perox flowcell. The use of a sheath stream in the flowcell allows a cell-by-cell
measurement of light scatter and absorption.
The hemoglobin colorimeter assembly ( 2 ) takes voltage readings that correspond
to the amount of transmitted light that passes through the reaction chamber. The
system uses the readings to derive the hemoglobin concentration.
The laser optical assembly ( 3 ) uses a laser diode light source. This optical
assembly is shared by the RBC/Plt, retic, and baso/lobularity channels. The use
of a sheath stream in the flowcell allows cell-by-cell measurement of low- and
high-angle light scattering and absorption.
Perox Optical Assembly
The perox optical assembly measures scattering and absorption of a tungsten
light beam as it passes through a stream of prepared white blood cells in a
flowcell.
A
D
E
G
I
B
C
F
H
A
Tungsten Lamp
D
Sample Stream
E Filter
G
Absorption PC Board
H Dark Field Stop