Beckman Coulter UniCel DxC 600, Instructions For Use Manual

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A13914AF
1-45
System Description
Cartridge Chemistry: Calibration Theory
1
Table 1.17 Calculation of Calibration Factors for Endpoint and Rate Chemistries
Type Formula
Nonblanked
Endpoint
Chemistries FOR HIGH CALIBRATOR LEVEL:
Reaction ABS = ABS
rep1
Reaction ABS = ABS
rep2
(ABS
rep1
+ ABS
rep2
) × 0.5 = ABS
avg
(hi)
FOR LOW CALIBRATOR LEVEL:
Reaction ABS = ABS
rep1
Reaction ABS = ABS
rep2
(ABS
rep1
+ ABS
rep2
) × 0.5 = ABS
avg
(lo)
Offset = Cal Set Point (hi) - [Cal Factor × ABS
avg
(hi)]
Sample values are calculated by the following equation:
(Reaction ABS × Cal Factor) + offset = sample value
IMPORTANT
For single point linear calibration, the low calibrator is a fixed zero point
and the offset is equal to zero.
Blanked Endpoint
Chemistries FOR HIGH CALIBRATOR LEVEL:
(Reaction ABS - Blank ABS) = Delta ABS
rep1
(Reaction ABS - Blank ABS) = Delta ABS
rep2
(Delta ABS
rep1
+ Delta ABS
rep2
) × 0.5 = Delta ABS
avg
(hi)
FOR LOW CALIBRATOR LEVEL:
(Reaction ABS - Blank ABS) = Delta ABS
rep1
(Reaction ABS - Blank ABS) = Delta ABS
rep2
(Delta ABS
rep1
+ Delta ABS
rep2
) × 0.5 = Delta ABS
avg
(lo)
Offset = Cal Set Point (hi) - [Cal Factor × Delta ABS
avg
(hi)]
Sample values are calculated by the following equation:
[(Reaction ABS - Blank ABS) × Cal Factor] + offset = sample value
IMPORTANT
For single point linear calibration, the low calibrator is a fixed zero point
and the offset is equal to zero.
E014416L.EPS
Cal Factor (Slope) =
ABS
avg
(hi) – ABS
avg
(lo)
Cal Set Point (hi) – Cal Set Point (lo)
E014417L.EPS
Cal Factor (Slope) =
Delta ABS
avg
(hi) – Delta ABS
avg
(lo)
Cal Set Point (hi) – Cal Set Point (lo)