Beckman Coulter UniCel DxC 600, Instructions For Use Manual

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A13914AF
8-18
User Defined Reagents
Exit Check Criteria
•
Calibrator target values must be unique -- no two calibrator levels can have the same analyte
target value (setpoint).
•
Models 1, 2, 3, 8 & 9 and DAT do not support negative setpoints.
•
If the math model is either Model 1 or Model 9, the number of calibrators must be five or six
levels only.
•
If the math model is Model 2, Model 3, or Model 8, the number of calibrators must be exactly six
levels.
•
If the math model is DAT, the number of calibrators must be exactly three levels. The middle
level defines the cutoff between positive and negative. The lowest and highest levels are used
to measure the reagent sensitivity.
•
If the math model has been specified as LINEAR, the number of cal levels must be 0, 1 or 2.
•
If the math model is DAT, the result units must be mA/min (rate) or mA (absorbance).
Blanking
•
The reagent blank end time must exceed the reagent blank start time by a minimum of one
processing cycle.
•
The blank absorbance high limit must be greater than the blank absorbance low limit.
•
The rate low limit cannot exceed the high limit for positive reaction direction chemistries.
•
The rate low limit must exceed the high limit for negative reaction direction chemistries.
Measurement
•
The reaction end-read time must exceed the reaction start-read time by a minimum of one
processing cycle.
•
The reaction absorbance high limit must be greater than the reaction absorbance low limit.
•
The usable range upper limit must be greater than the usable range lower limit.
Volumes
•
If a reagent injection (first, second and third) specifies a reagent component to be dispensed,
component A, B or C is specified, the corresponding dispense volume must be greater than zero.
Reagent volumes of 0 μL indicate a volume that is not set.
•
The sample volume must be greater than 0 μL.
•
The sum of all reagent volumes added prior to the reagent blank read must be greater or equal
to 200 μL.
•
The sum of sample volume and all reagent volumes must not exceed 330 μL.
•
The first, second and third reagent inject components must be from different compartments of
the reagent package.
•
The first reagent inject must be from a larger reagent compartment than either the second or
third reagent inject. The relative capacities of the reagent package compartments are:
A > B > C.