Beckman Coulter UniCel DxC 600, Instructions For Use Manual

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A13914AF
8-17
User Defined Reagents
Exit Check Criteria
8
Option 2
Prepare the reagent according to the manufacturer’s instructions. With a manual
spectrophotometer or automated instrument, obtain the delta absorbance per minute on at least
ten replicates of a normal or high normal sample at each of the appropriate wavelengths selected.
Average the delta absorbance per minute obtained at each wavelength. The extinction coefficient
at the secondary wavelength may be calculated by use of a factor or correction coefficient (R)
derived from the ratio of the delta absorbance per minute at the secondary and primary
wavelengths. The following formula may be used:
Exit Check Criteria
Introduction
When preparing to save a chemistry protocol after editing, the parameters are checked for
correctness, completeness, and consistency. Chemistry protocols that fail any Exit Check criteria
are not allowed to run. The following requirements specify the Exit Check criteria and parameters
presented for modification when the user elects to correct detected protocol errors.
Wavelengths
•
The primary wavelength must not be None .
•
The secondary wavelength must not be
None
.
•
The primary wavelength must be different from the secondary wavelength.
Calibration
•
The calibration time limit must be greater than zero hours if the number of calibrator levels is
greater than zero.
•
The sequence of calibrators must be in ascending target value order.
e
2
= e
1
× R
where:
e
2
= Extinction coefficient at the secondary wavelength
e
1
=
Extinction coefficient at the primary wavelength
a
a. The extinction coefficient of the chromophore at the primary wavelength is usually specified by the reagent manufacturer.
E01442 8 L.EPS
R =
Delta absorbance / min u te at primary wavelength
Delta absorbance / min u te at secondary wavelength