Beckman Coulter UniCel DxC 600, Instructions For Use Manual

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A13914AF
8-11
User Defined Reagents
Processing Parameters
8
Third Inject: Dispense Volume
The combined volume of all inject reagents must be between 200 and 327 μL.
Third Inject: Add Time
Add Time programs the reagent dispense time into the cuvette.
•
The Third Inject may be added a minimum of 8 seconds after reagent(s) and/or sample have
been pipetted. (Time is entered in increments of 1 second; however, the reagent is added at the
8-second interval closest to the time defined. Sample addition occurs at 1 second.)
•
A positive number indicates Third Inject reagent component is added after sample add.
•
Third Inject add time must be greater than Second Inject add time.
Sample Volume
Total volume of sample and reagents A, B, and C cannot exceed 330 μL. Volume is specified in
increments of 1 μL.
Blank Read Times
Blank read times indicate when the blank absorbance is to be read.
•
Time -180 to -1 is a reagent blank.
•
Time 1 is when sample is added to the cuvette.
• Time 1 to 720 is a sample blank.
•
Time is entered in 1-second intervals, but readings begin and end at the closest 8-second
interval.
•
Read times should not coincide with a pipetting cycle.
Table 8.20 Options for the Third Inject Dispense Volume
6 to 75 μL
Table 8.21 Allowable Entries for Add Time for the Third Inject
-172, 9 to 738 seconds
Table 8.22 Allowable Entries for Sample Volume
3 to 40 μL
Table 8.23 Blank Read Times
Allowable Entry
Start Read -180 to 1704 seconds, except 0
End Read -180 to 1720 seconds, except 0