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1
Installation
Installing the Column the First Time
Thermo Scientific
TRACE 1300 and TRACE 1310 Hardware Manual
61
b. Connect the flowmeter to the dedicated fitting on the column-flowmeter connector.
c.
If your GC is equipped with the touch screen as user interface, select
Back
or
Front
Column
icon in the
Configuration
menu, otherwise perform the Column
Evaluation through the Chromatography Data System by selecting the proper
function.
d. Select the column and input the physical characteristics of the column.
e.
If a pre-/post-column is present, set the length and nominal internal diameter of the
pre-/post-column in the same valid ranges for the column. The following two lines
are added to the menu.
f.
According to the physical characteristics of the column, the system calculates and
displays the relevant Column K-factor.
g. Start column evaluation. At the end of the routine, a message will indicate that
evaluation was successful.
h. Expect a K-factor of approximately 0.7 – 0.9 for a 15 m, 0.25 mm ID column
(1.3 – 2.0 for a 30 m, 0.25 mm ID column). If the column does not report a K-factor
within this range or within 0.1 units of the previous stored value, check for a leak or
broken column using the leak detector. The K-factor is a measured resistance for the
column. A K-factor that is too low may indicate a leak in the system, while a K-factor
that is too high may indicate a blockage.
8. Disconnect the column-flowmeter.
a.
Disconnect the column from the column-flowmeter connector.
b. Remove the clear plastic component, including its fittings, from the oven and set it
aside.
c.
Close the GC door.
9. Condition the column.
The column must be conditioned before inserting it into the detector.
Column conditioning consists of passing a carrier gas flow through the column heated at
a programmed temperatures as described in the
column manufacturer’s instructions
.
In case the column does not have any column conditioning instructions, perform the
column conditioning by setting a final temperature up to 10 °C - 20 °C below its
recommended maximum temperature.
Note
For the most reproducible results, you should conduct a more detailed
column evaluation. However, the following steps, while recommended, are not
required.
CAUTION
When performing column conditioning, the column should be connected only
to the injector leaving the column outlet disconnected to avoid the possibility of
contamination of the detector. Do not use hydrogen as the carrier for conditioning! It
could vent into the oven and present an explosion hazard.
Содержание TRACE 1300
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