Thermo Scientific TRACE 1300, Руководство по оборудованию

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2
Performing Routine Maintenance
Replacing a Column
Thermo Scientific TRACE 1300 and TRACE 1310 Hardware Manual 77
b. Unwind the column enough to easily connect its ends to the injector and the
detector.
c. Wipe about 100 mm (4 in.) of the column with a tissue soaked in methanol.
d. Use a scoring wafer to score and break the column outlet about 2.5cm (1 in.) from
the end. Use a magnifying glass to check for an even, flat cut. Repeat if necessary.
e. Insert the column through the proper detector retaining nut and ferrule (open end
up). Wipe the column again with a tissue soaked in methanol.
f. For FID , NPD , TCD , ECD , and FPD , position the column so that the end of the
column extends the proper distance above the end of the ferrule as reported in
Table 13 . For PDD see the instruction described at the step g on page 77 .
i. For FID , NPD , and TCD , insert the column into the detector, paying attention
to not force it further. Finger-tighten the retaining nut, then withdraw the
column
2-3 mm . Tighten the retaining nut an additional a quarter turn.
ii. For ECD and FPD , insert the notched septum on the column to hold the
retaining nut in this position. Thread the retaining nut into the detector but do
not tighten. Adjust the column position so that the septum contact the bottom
of the retaining nut. Finger-tighten the retaining nut until it starts to grip the
column plus a quarter turn.
iii. Remove the notched septum from the column.
g. For PDD the column must enter 136 mm into the pre-installed capillary column
adapter.
i. Make a mark on the column 136 mm from the end.
WARNING-BURN HAZARD: The injector, detectors, oven, and transfer line may be hot.
Allow them to cool to room temperature before touching them.
Note Wear clean, lint- and powder-free gloves when you handle the column and
injector ferrule.
Tip Slide a notched septum on the column before the detector retaining nut to
make it easier to measure the proper distance between the bottom nut and end of
the column.
Table 13. Column Insertion Depth For FID, NPD, TCD, ECD, FPD, and PDD Detectors
Detector Column Insertion Depth
FID, NPD, and TCD Insert the column as far as goes and
withdrawn about 2 -3 mm
ECD 23 mm
FPD 125 mm
PDD 136 mm