Thermo Scientific TRACE 1300, Руководство по оборудованию

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2
Performing Routine Maintenance
Replacing a Column
76 TRACE 1300 and TRACE 1310 Hardware Manual Thermo Scientific
g. Start column evaluation. At the end of the routine, a message will indicate that
evaluation was successful.
h. Expect a K-factor of approximately 0.7 – 0.9 for a 15 m, 0.25 mm ID column
(1.3 – 2.0 for a 30 m, 0.25 mm ID column). If the column does not report a K-factor
within this range or within 0.1 units of the previous stored value, check for a leak or
broken column using the leak detector. The K-factor is a measured resistance for the
column. A K-factor that is too low may indicate a leak in the system, while a K-factor
that is too high may indicate a blockage.
7. Disconnect the column flowmeter.
a. Disconnect the column from the column flowmeter connector.
b. Remove the clear plastic component, including its fittings, from the oven and set it
aside.
c. Close the GC door.
8. Condition the column.
The column must be conditioned before inserting it into the detector.
Column conditioning consists of passing a carrier gas flow through the column heated at
a programmed temperatures as described in the
column manufacturer’s instructions .
In case the column does not have any column conditioning instructions, perform the
column conditioning by setting a final temperature up to 10 °C - 20 °C below its
recommended maximum temperature.
a. Run the slow temperature program that is recommended by the manufacturer.
9. Connect the column to the detector inside the GC.
a. Lower the oven temperature to 30 °C and allow it to cool.
Note For the most reproducible results, you should conduct a more detailed
column evaluation. However, the following steps, while recommended, are not
required.
CAUTION When performing column conditioning, connect the column only to the
injector leaving the column outlet disconnected to avoid the possibility of contamination
of the detector.
Do not use hydrogen as the carrier for conditioning! It could vent into the oven and
present an explosion hazard.
INSTRUMENT DAMAGE: Never exceed the column manufacturer’s maximum operating
temperature.