Thermo Scientific TRACE 1300, Руководство по оборудованию

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2
Performing Routine Maintenance
Replacing a Column
74 TRACE 1300 and TRACE 1310 Hardware Manual Thermo Scientific
e. Position the column so that the end of the column extends the proper distance above
the end of the ferrule as reported in
Table 12 .
f. Insert the notched septum on the column to hold the retaining nut at this position.
Thread the retaining nut into the injector but do not tighten.
g. Adjust the column position so that the septum contact the bottom of the retaining
nut.
h. Finger-tighten the retaining nut until it starts to grip the column plus a quarter turn.
i. Remove the notched septum from the column.
4. Setup the GC parameters.
a. Set the oven and injector temperature to 50 °C.
b. Use the column flowmeter connector to verify that there is flow through the column.
If you do not have a flowmeter, dip the column outlet in a small vial of methanol.
Bubbles indicate there is flow through the column. If there is no flow, check that the
carrier gas is on, the GC inlet is pressurized, and the column is not plugged. If there is
still no flow, consult the Troubleshooting section or contact Technical Support.
c. Allow the column to purge for at least 10 minutes.
5. Perform a column leak check.
Tip Slide a notched septum on the column before the injector retaining nut to
make it easier to measure the proper distance between the nut and end of the
column.
Table 12. Column Insertion Depth For SSL, SSLBKF, HeS-S/SL, PTV, PTVBKF, and GSV
Injectors
Injector Column Insertion Depth
SSL and HeS-S/SL • 5 mm (splitless)
• 5 mm (He saver
• 10 mm (split)
SSLBKF • 5 mm (splitless)
• 10 mm (split)
PTV • 30 mm
• As far as possible into the bottom
when the PTV is used as an
On-Column injector.
PTVBKF • 30 mm
GSV • Insert the column as far as goes and
withdrawn about 2 -3 mm