Beckman Coulter CEQ 8000, User Manual

Page: 225 / 382

Creating a Reference File
User’s Guide 211
Viewing Reference Amino Acid Translations
When the system generates the comparison, it translates the nucleotide sequence of the
reference file into an amino acid sequence and displays it above the reference base
sequence line. From the beginning of the reference, the bases are separated into groups
of three nucleotides (codons). Each group is translated into an amino acid which is
displayed above the codon. Each codon will be shaded to show its boundaries. (If an
inserted space exists, signified by a dash “–,” the space will be included in the codon.)
The amino acid designation is left-justified above the codon. If two amino acids are
possible, the letters designating the possible amino acids will be displayed. If more
than two possible amino acids are detected at any location, an asterisk is displayed
instead.
You may show or hide the reference amino acid sequence by clicking on the
View
menu, and then on
Reference Amino Acid Translation
. The reference amino acid
translation will be shown or hidden, depending on whether the item is checked or
unchecked.