Beckman Coulter CEQ 8000, User Manual

Page: 172 / 382

Sequence Analysis Procedures
158 CEQ™ 8000 Genetic Analysis System
Height Ratio
Use this parameter to define the minimum percent of the primary peak’s height that a
second peak falling within the defined range must be to be considered a heterozygous
peak. The range is 0 to 100% and the default is 30%.
Sensitivity
Use this parameter to define the sensitivity of the system when looking at the
sharpness of the peak. A high value leads to a high degree of sensitivity and will detect
“peaks” that are not very well formed, i.e., “blips” in the baseline. A lower value will
lead to a low degree of sensitivity and will not detect “peaks” unless they are
well-define and are shaped more like a DNA fragment peak. The range is 0 to 1.0 and
the default is 0.25.
Range
This value defines the nucleotide range in the called sequence in which the system will
look for heterozygous peaks. The system will not look for heterozygous peaks outside
this nucleotide range. The range max is dependent upon the length of the analyzed
sequence for the current sample.
Range From (Minimum)
If
Range From
is zero, detection will start at the first called base. The range is 0 to
2000 and the default is 50.
Range to (Maximum)
If
Range to
is zero, the algorithm will use the
Range End
value.
If both
Range From
and
Range to
are zero, detection will continue to the end of
the called base sequence.
If
Range End
is zero, the system will use the
Range to
value.
If both
Range From
and
Range End
have a non-zero value, the algorithm will
choose the earlier of the two values.
Range End (“or to”)
This item specifies how many bases, before the last called base, to stop looking for
heterozygotes.
If the value in
Range End
is zero, the algorithm will use the
Range to
value.
If both
Range End
and
Range to
are zero, detection will continue to the end of the
called base sequence.
If
Range to
is zero, the system will use the
Range End
value.
If both
Range to
and
Range End
have a non-zero value, the algorithm will choose
the earlier of the two values.
If a value is outside of an expected range, a message box will be dis-
played requesting that a valid value be entered.