Beckman Coulter CEQ 8000, User Manual

Page: 280 / 382

Fragment Analysis Procedures
266 CEQ™ 8000 Genetic Analysis System
The Filter Sets applied to the Result Set data (2nd Level Filtering) are
different from the Filter Sets applied to the Fragment List data (3rd
Level Filtering). To the Result Set you apply filters to an entire sample
which may contain numerous fragments. To the Fragment List you
apply filters to individual fragments that have been identified within
the various samples. See the “Fragment List” section of this manual or
Online Help for more information.
Applying Exclusion Filters to the Fragment List
The purpose of the Filter Set is to exclude any fragments that do not meet the criteria
of the Study and to include fragments that do.
Refer to the Online Help for information and examples regarding the
application of various filters to the Fragment List.
There are many filters available and each must be applied using an operator and a
value. Any number of filters can be applied to a result set and the filter sets can be
saved for future use.
You can assign filter sets to filter out fragments in which no confidence interval was
estimated, those with asymmetric peaks, stutter peaks, etc.
The end result of this “third level” filtering is to leave you only sample data with
fragments that you are certain of and to save you the time of “weeding out” inaccurate
fragment data later in the analysis.
Available Exclusion Filters
All of the exclusion filters can be found in the drop-down list box available in the
Name
column of the
Filter Set
area. Once you have selected the name of the filter to
use, you must select an
operator
(u, <, >=, etc.) and a
value
to be applied to
the filter.
Refer to the Online Help for detailed descriptions of the available
exclusion filters and their use.
Customizing the Fragment List
The components of the Fragment List are user-defined and can be modified through
commands available in the right-click menus. These commands allow you to select
specific columns for display in the fragment list, customize the arrangement of
fragment list information and modify several other parameters of the fragment list.
Information regarding use of the Column Selector window, the
Column Sort window and other display parameters can be found in the
“Customizing the Fragment List” sections of the Online Help.