Beckman Coulter CEQ 8000, User Manual

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Analyzing Sequence Data
User’s Guide 157
Heterozygote Detection Tab
Select the
Heterozygote Detection
tab as shown in Figure 95 to define the
parameters the system will use to detect heterozygotes. These parameters will only be
used if the
Detect Heterozygote
option is selected in the General tab.
Figure 95: Heterozygote Detection Tab
Detect Heterozygotes
When this item is checked, the analysis will include heterozygote detection based on
the remaining parameters specified in the
Heterozygote Detection tab.
When the
Detect Heterozygotes
check box is checked, the
Call Threshold
is
disabled (grayed) but the current Call Threshold value remains in the text box. The
system will use a value of zero for the Call Threshold and will not call “N’s” when
attempting to detect heterozygotes.
% of Average Peak Spacing
Use this parameter to determine the range around each peak that the system will search
for a possible heterozygous peak. The system will determine the local peak to peak
spacing using an average of the spacing for a 10 base range around the base of interest.
The system will then find the specified percent (e.g., 70%) of the local peak to peak
spacing and look in that range from the center of each peak for a possible
heterozygous peak. For instance, if a value of 70% is entered, the system will look
35% of the average peak spacing to the right and 35% to the left for additional peaks.
The range is 0 to 100% and the default is 70%.