Beckman Coulter CEQ 8000, User Manual

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User’s Guide 237
6. The
+/- Window
field allows you to input a variable quantity to be considered
when identifying the reference peak.
• For example , if you listed the size of your reference peak as 90
nucleotides, you may enter a value of '1' in the +/- Window field.
• This will compensate for any variability that might arise in identification of the
reference peak.
•
This field can be either
+/- nucleotides
(when Size is selected) or
+/-
minutes
(when Time is selected).
7. Enter the known quantity of your reference peak in the
Amount
field.
• This value must be entered into
exponential notation
.
8. Select the
units
for the quantity entered into the
Amount
field from the
drop-down list box.
• Options are
moles (mol)
,
grams (g), molarity (M)
or
(g/L)
.
Fragment Analysis Parameters - STR Locus Tags tab
This tab allows you to select the locus tags that will be used for allele identification
with the parameter set being configured. Selections can be made from a list of
available STR locus tags previously configured for analysis. This tab also gives you
access to the
STR Locus Tag Editor
, where you can edit existing or create new
STR locus tags to meet the specific requirements of your samples.
Locus tags are used to define a particular locus of interest. Each available locus tag has
been configured with a specific set of parameters that define the manner in which the
system will identify detected fragments as allele fragments. These parameters include
the fragment size range, repeat unit length, the dye to be used and an allele list
associated with the locus tag.
When a locus tag is selected and an analysis performed, the system identifies the
alleles present within the locus region using the estimated size of the fragments and
the allele list.
The system will estimate sizes only if a size standard is used (see the
“Analysis Method tab” section) and will call alleles only if an allele list
exists for the selected locus tag (see the “Locus Tag Editor” section).
Although the STR Locus Tags tab is similar in appearance and
functionality to the SNP Locus Tags tab, the two types of Locus Tags
are used for different purposes. STR Locus Tags use size to define allele
fragments having a single specified dye label in a specified size range.
SNP Locus Tags use multiple dye labels for fragments of a single
specified size to define specific SNP variants. Additional information
can be found on the following pages and in Online Help.