Beckman Coulter CEQ 8000, User Manual

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Hardware
User’s Guide 5
Sample Plate
The Sample Plate (Figure 5) is used to hold the sample(s) for separation. The plate is a
V-bottom, thermal cycler-compatible, polypropylene plate containing 96 wells (8-rows
x 12-columns). The wells have a 200µL volume capacity.
Figure 5: Sample Plate
Buffer Plate with Buffer Evaporation Cover
The Buffer Plate (Figure 6) is used to hold the DNA separation buffer used during a
sample run. The plate is a flat-bottom, polystyrene, non-sterile plate containing 96
wells (8-rows x 12-columns). An evaporation cover, placed over the Buffer Plate, is
used to maintain the proper level of buffer (250-300 µL) in the Buffer Plate by
preventing evaporation of the buffer. The cover slips over the Buffer Plate position of
the Sample Transport. As the CEQ advances through the Sample Plate during a run,
the evaporation cover is pushed back just far enough to expose the next row of buffer
wells to be used for the next sample set run.
Figure 6: Buffer Evaporation Cover and Buffer Plate
Wetting Tray
The Wetting Tray (Figure 7) is used to immerse the ends of the capillaries in deionized
water. When properly filled, the capillaries can be maintained for approximately seven
days in the Wetting Tray without attention. During continuous use of the CEQ System,
the Wetting Tray should be replenished with deionized water after a 96-well
microplate has been run or prior to a sample plate run. The wetting tray is also used to
rinse capillary tips and provide a receptacle for separation medium purged from the
capillaries.
900497e.AI
900498e.AI
900496E.AI
900496e.AI
900498e.A1
Buffer Evaporation Cover
Buffer Plate