Beckman Coulter CEQ 8000, User Manual

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Fragment Analysis Procedures
268 CEQ™ 8000 Genetic Analysis System
Bin Analysis
“Binning” is a one-dimensional clustering, by size, of fragments compiled from
multiple samples. Bin analysis is used to generate an allele list that is representative of
the population being studied. This is particularly useful when establishing a database
of information on alleles and possible anomalies that are associated with them (such as
imperfect repeats).
Binning of alleles from multiple samples allow the user to create a new allele list or
update an existing allele list for a locus tag based on the collection of fragment results.
When bin analyses are performed, the system groups fragments by size to form “bins”
characterized by an average apparent size and standard deviation of the fragments in
the bin.
Setting up a Bin Analysis
A result set must exist in order to create a bin analysis. The result set could be a
previously saved Study or a new Study created solely for the purpose of binning. As
long as the result set is open you can begin a bin analysis.
• From the
Analysis
menu, select the
New Binning Analysis
command.
•
Bin Analysis
can also be accessed from the
Analyses
tab of the
Study
Explorer
.
• The
New Binning Analysis
window will be displayed.