Beckman Coulter CEQ 8000, User Manual

Page: 169 / 382

Analyzing Sequence Data
User’s Guide 155
Check the
Use Final Values as Initial Values
check box to use the final values to
re-compute the color calibration the next time the data is analyzed. Otherwise the
system will use the initial values to re-compute the color calibration. (This option is
not available if accessed from the Parameters Used to Compute Sequence menu option
under the View menu.)
Initial Data Detection
Select the Initial Data Detection tab to define the parameters that the system will use to
define the start of usable data when an Analysis Start time, on the
General tab, is not
entered. See Figure 94.
The default settings are different when the
<200 Bases
option is selected. The
default settings are:
Threshold: 40
Delay: 0.20
Signal to Noise: 7.00
Minimum Duration: 2.00
Figure 94: Initial Data Detection tab
Threshold
The percent threshold is the percent of the maximum of the energy profile of all the
data above which the signal must rise before start of data is detected. The data must
stay above the threshold for the amount of the minimum duration without gaps lasting
greater than 21 seconds. The range is 0 to 100 and the default is 40.