Beckman Coulter CEQ 8000, User Manual

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Sequence Analysis Procedures
178 CEQ™ 8000 Genetic Analysis System
Analysis Log
The system will log all the steps used in performing the base-calling routine on the raw
data in the Analysis Log. For example: the system will log a message when it is unable
to find the end of PCR product data.
The Analysis Log may be viewed to see if the system was unable to find the end of
PCR data. The following message will appear in the Analysis Log: “Analyzed Data:
Cannot compute stop time. Using data stop time of xxx.xx minutes”, as shown in
Figure 111.
Figure 111:Analysis Log
Occasionally, the system will not be able to find the end of PCR product data, even if
the PCR Product option is selected. You will be able to determine if this is the case by
reviewing the analysis log, and you can use this information in future analysis of the
same sample.
To view the Analysis Log:
1. Open the sequence results you would like to review.
If the sample data has not yet been analyzed, analyze the sample data set
using the desired analysis parameters. Make sure that the PCR Product
check box is selected and that 0.0 is entered for both the Analysis Start and
Stop times.
2. Select
View | Analysis Log
to view the analysis log for the sequence result.
3. The Analysis Log will be displayed in a pane at the bottom of the window.