Beckman Coulter CEQ 8000, User Manual

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User’s Guide 233
Fragment Analysis Parameters - Analysis Method tab
This tab allows you to specify the size standard and model to be applied to the
calibration curve. The model selected should be the best fit for the standard, as it is
used to generate the calibration curve, which estimates sizes for the unknown
fragments.
Figure 136:Fragment Analysis Parameters window - Analysis Method tab
Size standards are sets of DNA fragments of known sizes that are run with a sample
and are used to create the calibration curve. This calibration curve is then used to relate
the known fragment size to either migration time or mobility in order to estimate the
size of the unknown fragment(s).
When creating a new size standard, there are two requirements that help the system to
find the standards and make correct associations between the fragment peaks and the
fragment list. Refer to Online Help for information on creating size standards.
1. Fragments in the larger half of the size range should be spaced uniformly.
• For example, the peaks in the range of 200 to 400 nucleotides of the
SizeStandard-400 are 20 nucleotides apart.
2. Fragments in the smaller half of the size range should include a set of at least three
fragments that are spaced approximately half the distance of those that are in the
larger fragment size range.