Beckman Coulter CEQ 8000, User Manual

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Creating a Reference File
User’s Guide 207
CEQuence Investigator Procedures
Creating a Reference File
The reference file is a text file that can be created using notepad or any other text
editor. It may be composed of one or two sections; the sequence and, optionally,
specific nucleotide or amino acid mutations. To create the file enter the text of the
sequence starting in the first line of the text editor. The sequence text may be broken
up into several lines.
To create a reference file from the Sequence Analysis Module, export the
data in *.seq format or choose *.txt with only Result Output checked.
To enter known protein or nucleotide mutations, perform the following:
1. On the line below the reference sequence, type an open bracket, then “KNOWN
AA MUTATIONS” and/or “KNOWN BASE MUTATIONS” then a closed
bracket.
2. On the next line, type the current reference amino acid or base, the position
number using the amino acid or base number, then the expected amino acid or
base change.
A sample reference is shown below.
Reference Sequence TCCTGTC
Amino Acid Mutations [KNOWN AA MUTATIONS]
Known Amino Acid Notation Q2K
Base Mutations [KNOWN BASE MUTATIONS]
Known Base Notation A30C